Comparison of human immunodeficiency virus antigens as stimulants for lymphocyte proliferation assays.

Published

Journal Article

CD4 proliferative responses to the human immunodeficiency virus (HIV) type 1 (HIV-1) p24 (gag) antigen inversely correlate with the plasma viral load in HIV-infected subjects who control viral replication without antiretroviral therapy. Use of a single HIV-1 protein to assess CD4 proliferative responses may not reflect the global response to this pathogen. We compared the abilities of HIV p24 and gp120 antigens from two different vendors, an inactivated whole HIV-1 MN virion preparation and an HIV-1E culture supernatant antigen, to elicit proliferative responses in HIV-seropositive and HIV-seronegative donors. Peripheral blood mononuclear cells from 12 HIV-seropositive donors (each with HIV-1 loads <4,000 copies/ml of plasma, >350 CD4 T lymphocytes/mm(3), and no antiretroviral therapy) and 15 HIV-seronegative donors were assessed with multiple concentrations of each stimulant by standard lymphocyte proliferation assays. Wide variations in response rates were found, with zero, three, five, and eight individuals demonstrating stimulation indices of >3 for the HIV culture antigen supernatant, gp120, p24, and inactivated whole-virus preparations, respectively. These results suggest that the use of the inactivated whole virus resulted in a more sensitive assay for detection of CD4 T-lymphocyte function in HIV-infected subjects.

Full Text

Duke Authors

Cited Authors

  • Schmitz, JL; Denny, TN; Garcia, A; Lathey, JL; Adult and Pediatric AIDS Clinical Trials Group Immunology Laboratory Subcommittees,

Published Date

  • May 2002

Published In

Volume / Issue

  • 9 / 3

Start / End Page

  • 525 - 529

PubMed ID

  • 11986255

Pubmed Central ID

  • 11986255

International Standard Serial Number (ISSN)

  • 1071-412X

Digital Object Identifier (DOI)

  • 10.1128/cdli.9.3.525-529.2002

Language

  • eng

Conference Location

  • United States