Karyotyping of Cryptococcus neoformans as an epidemiological tool.

Journal Article (Journal Article)

Karyotyping of Cryptococcus neoformans var. neoformans can be used as an epidemiological tool for C. neoformans infections. In this study of over 40 isolates from both clinical and environmental sources, 90% had a unique chromosome banding by pulsed-field electrophoresis. There was no conserved pattern associated with body site of infection, geographical location of the isolate, or human immunodeficiency virus status. Karyotypes of individual isolates remained stable during both in vitro passage and in vivo infections. Karyotype was used to exclude the possibility of nosocomial spread of C. neoformans in one clinical situation and supported relapse in two other cases. Because of its variable sizes between isolates, karyotyping of C. neoformans is a convenient method for molecular identification of different strains.

Full Text

Duke Authors

Cited Authors

  • Perfect, JR; Ketabchi, N; Cox, GM; Ingram, CW; Beiser, CL

Published Date

  • December 1993

Published In

Volume / Issue

  • 31 / 12

Start / End Page

  • 3305 - 3309

PubMed ID

  • 8308124

Pubmed Central ID

  • PMC266409

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.31.12.3305-3309.1993


  • eng

Conference Location

  • United States