Identification of Cryptococcus neoformans temperature-regulated genes with a genomic-DNA microarray.

Published

Journal Article

The ability to survive and proliferate at 37 degrees C is an essential virulence attribute of pathogenic microorganisms. A partial-genome microarray was used to profile gene expression in the human-pathogenic fungus Cryptococcus neoformans during growth at 37 degrees C. Genes with orthologs involved in stress responses were induced during growth at 37 degrees C, suggesting that a conserved transcriptional program is used by C. neoformans to alter gene expression during stressful conditions. A gene encoding the transcription factor homolog Mga2 was induced at 37 degrees C and found to be important for high-temperature growth. Genes encoding fatty acid biosynthetic enzymes were identified as potential targets of Mga2, suggesting that membrane remodeling is an important component of adaptation to high growth temperatures. mga2Delta mutants were extremely sensitive to the ergosterol synthesis inhibitor fluconazole, indicating a coordination of the synthesis of membrane component precursors. Unexpectedly, genes involved in amino acid and pyrimidine biosynthesis were repressed at 37 degrees C, but components of these pathways were found to be required for high-temperature growth. Our findings demonstrate the utility of even partial-genome microarrays for delineating regulatory cascades that contribute to microbial pathogenesis.

Full Text

Duke Authors

Cited Authors

  • Kraus, PR; Boily, M-J; Giles, SS; Stajich, JE; Allen, A; Cox, GM; Dietrich, FS; Perfect, JR; Heitman, J

Published Date

  • October 2004

Published In

Volume / Issue

  • 3 / 5

Start / End Page

  • 1249 - 1260

PubMed ID

  • 15470254

Pubmed Central ID

  • 15470254

International Standard Serial Number (ISSN)

  • 1535-9778

Digital Object Identifier (DOI)

  • 10.1128/EC.3.5.1249-1260.2004

Language

  • eng

Conference Location

  • United States