Alpha1-adrenergic receptors in human spinal cord: specific localized expression of mRNA encoding alpha1-adrenergic receptor subtypes at four distinct levels.

Published

Journal Article

alpha1-Adrenergic receptors (alpha1ARs) are important in lower urinary tract syndromes such as benign prostatic hypertrophy and bladder irritability. Spinal cord alpha1ARs have been postulated to play a role in modulating these diseases, yet alpha1AR subtype (alpha1a, alpha1b, alpha1d) neuronal localization in human spinal cord has not been described. We therefore tested the hypothesis that alpha1AR subtype distribution varies according to specific spinal cord tract and level. In situ hybridization was performed to identify cell bodies containing alpha1AR subtype mRNA at four levels of human spinal cord (cervical enlargement, thoracic, lumbar, sacral). alpha1AR mRNA is present in ventral gray matter only (ventral>dorsal; sacral>lumbar=thoracic>cervical). Signaling cell bodies were detected in anterior horn motor neurons at all levels; dorsal nucleus of Clarke and intermediolateral columns in cervical enlargement, thoracic and lumbar spinal cord regions; and parasympathetic nucleus in sacral spinal cord. Although all three alpha1AR subtypes are present throughout human spinal cord, alpha1d mRNA predominates overall. If confirmed at a protein level, these findings may contribute to the development of new therapeutic strategies in the treatment of several human diseases.

Full Text

Duke Authors

Cited Authors

  • Smith, MS; Schambra, UB; Wilson, KH; Page, SO; Schwinn, DA

Published Date

  • January 8, 1999

Published In

Volume / Issue

  • 63 / 2

Start / End Page

  • 254 - 261

PubMed ID

  • 9878769

Pubmed Central ID

  • 9878769

International Standard Serial Number (ISSN)

  • 0169-328X

Digital Object Identifier (DOI)

  • 10.1016/s0169-328x(98)00287-3

Language

  • eng

Conference Location

  • Netherlands