Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts.
Journal Article (Review)
Beta-adrenergic receptor signaling, desensitization, and downregualtion are fundamental mechanisms that contribute to both normal and altered myocardial function. The development of pharmacological and biochemical assays has provided the ability to measure alterations both in adrenergic receptor density and the subsequent coupling of adrenergic receptors downstream effectors, namely, adenylyl cyclase. Furthermore, transthoracic echocardiography of the murine heart has provided insight into changes in cardiac physiology that accompany altered adrenergic receptor signaling. The protocols described within this chapter provide the means to quantify beta-adrenergic density, measure adenylyl cyclase activity, and evaluate cardiac hypertrophy to better understand the mechanisms responsible for cardiac hypertrophy and heart failure.
Full Text
Duke Authors
Cited Authors
- Wolf, MJ; Tachibana, H; Rockman, HA
Published Date
- 2005
Published In
Volume / Issue
- 112 /
Start / End Page
- 353 - 362
PubMed ID
- 16010029
Pubmed Central ID
- 16010029
International Standard Serial Number (ISSN)
- 1543-1894
Digital Object Identifier (DOI)
- 10.1385/1-59259-879-x:353
Language
- eng
Conference Location
- United States