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Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts.

Publication ,  Journal Article
Wolf, MJ; Tachibana, H; Rockman, HA
Published in: Methods Mol Med
2005

Beta-adrenergic receptor signaling, desensitization, and downregualtion are fundamental mechanisms that contribute to both normal and altered myocardial function. The development of pharmacological and biochemical assays has provided the ability to measure alterations both in adrenergic receptor density and the subsequent coupling of adrenergic receptors downstream effectors, namely, adenylyl cyclase. Furthermore, transthoracic echocardiography of the murine heart has provided insight into changes in cardiac physiology that accompany altered adrenergic receptor signaling. The protocols described within this chapter provide the means to quantify beta-adrenergic density, measure adenylyl cyclase activity, and evaluate cardiac hypertrophy to better understand the mechanisms responsible for cardiac hypertrophy and heart failure.

Duke Scholars

Published In

Methods Mol Med

DOI

ISSN

1543-1894

Publication Date

2005

Volume

112

Start / End Page

353 / 362

Location

United States

Related Subject Headings

  • Signal Transduction
  • Receptors, Adrenergic, beta
  • Radioligand Assay
  • Myocardium
  • Mice
  • Ligands
  • Hemodynamics
  • Echocardiography
  • Cell Membrane
  • Cardiomegaly
 

Citation

APA
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ICMJE
MLA
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Wolf, M. J., Tachibana, H., & Rockman, H. A. (2005). Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts. Methods Mol Med, 112, 353–362. https://doi.org/10.1385/1-59259-879-x:353
Wolf, Matthew J., Hideo Tachibana, and Howard A. Rockman. “Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts.Methods Mol Med 112 (2005): 353–62. https://doi.org/10.1385/1-59259-879-x:353.
Wolf MJ, Tachibana H, Rockman HA. Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts. Methods Mol Med. 2005;112:353–62.
Wolf, Matthew J., et al. “Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts.Methods Mol Med, vol. 112, 2005, pp. 353–62. Pubmed, doi:10.1385/1-59259-879-x:353.
Wolf MJ, Tachibana H, Rockman HA. Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts. Methods Mol Med. 2005;112:353–362.

Published In

Methods Mol Med

DOI

ISSN

1543-1894

Publication Date

2005

Volume

112

Start / End Page

353 / 362

Location

United States

Related Subject Headings

  • Signal Transduction
  • Receptors, Adrenergic, beta
  • Radioligand Assay
  • Myocardium
  • Mice
  • Ligands
  • Hemodynamics
  • Echocardiography
  • Cell Membrane
  • Cardiomegaly