Pegvisomant interference in GH assays results in underestimation of GH levels.

Published

Journal Article

Pegvisomant use in acromegaly negates the use of GH levels to monitor disease activity. To achieve antagonism, plasma concentrations must be approximately 1000-fold greater than GH which with the high homology between the peptides makes GH measurement a challenge when pegvisomant is present.We investigated the effect of pegvisomant on GH measured using commercially available assays.Pooled serum samples with GH concentrations <0.38, 3.85 and 7.69 microg/l were spiked with increasing pegvisomant concentrations (9000-494 000 microg/l). Samples were analysed by the Nichols Advantage, DPC Immulite 2000, Diasorin IRMA, Beckman Access Dxl, Tosoh AIA and Wallac Delfia assays.With baseline GH <0.38 microg/l measured levels were <0.38 in all assays except Nichols, Diasorin and Beckman where GH peaked at 1.5, 9.6 and 17.7 micarog/l respectively at low pegvisomant concentrations, falling thereafter. With the other two samples, measured GH levels progressively fell with increasing pegvisomant concentrations, except the Beckman assay where an increase (30.8 microg/l) was seen at a pegvisomant concentration of 9000 microg/l; and Diasorin and Tosoh where smaller increases were seen at lower pegvisomant concentrations, levels gradually falling thereafter.The presence of pegvisomant resulted in artefactually low measured GH in most assays. We speculate this fall is due to assay antibody-binding pegvisomant, reducing the amount of available antibody to bind actual GH thereby producing less sandwich formation: the 'high-dose hook' effect. In most assays, this effect is modest and results in lower GH, but the level of interference makes them unsuitable for studies on the influence of pegvisomant on GH neuroregulation.

Full Text

Cited Authors

  • Paisley, AN; Hayden, K; Ellis, A; Anderson, J; Wieringa, G; Trainer, PJ

Published Date

  • March 2007

Published In

Volume / Issue

  • 156 / 3

Start / End Page

  • 315 - 319

PubMed ID

  • 17322491

Pubmed Central ID

  • 17322491

Electronic International Standard Serial Number (EISSN)

  • 1479-683X

International Standard Serial Number (ISSN)

  • 0804-4643

Digital Object Identifier (DOI)

  • 10.1530/eje.1.02341

Language

  • eng