A low-cost, portable, and quantitative spectral imaging system for application to biological tissues.

Published

Journal Article

The ability of diffuse reflectance spectroscopy to extract quantitative biological composition of tissues has been used to discern tissue types in both pre-clinical and clinical cancer studies. Typically, diffuse reflectance spectroscopy systems are designed for single-point measurements. Clinically, an imaging system would provide valuable spatial information on tissue composition. While it is feasible to build a multiplexed fiber-optic probe based spectral imaging system, these systems suffer from drawbacks with respect to cost and size. To address these we developed a compact and low cost system using a broadband light source with an 8-slot filter wheel for illumination and silicon photodiodes for detection. The spectral imaging system was tested on a set of tissue mimicking liquid phantoms which yielded an optical property extraction accuracy of 6.40 +/- 7.78% for the absorption coefficient (micro(a)) and 11.37 +/- 19.62% for the wavelength-averaged reduced scattering coefficient (micro(s)').

Full Text

Duke Authors

Cited Authors

  • Fu, HL; Yu, B; Lo, JY; Palmer, GM; Kuech, TF; Ramanujam, N

Published Date

  • June 2010

Published In

Volume / Issue

  • 18 / 12

Start / End Page

  • 12630 - 12645

PubMed ID

  • 20588390

Pubmed Central ID

  • 20588390

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.18.012630

Language

  • eng