Molecular analysis and protein processing in late-onset Pompe disease patients with low levels of acid α-glucosidase activity.
Journal Article
Introduction
Pompe disease (glycogen storage disease type II, acid maltase deficiency) is caused by deficiency of lysosomal acid α-glucosidase (GAA). A few late-onset patients have been reported with skin fibroblast GAA activity levels of <2%.Methods
We measured GAA activity in skin fibroblasts from 101 patients with late-onset Pompe disease. Whenever possible, we performed Western blot analysis and correlated the results with GAA activity and GAA gene mutations.Results
Thirteen patients (13%) had skin fibroblast GAA activity of <1% of normal. Although there was wide genetic heterogeneity, none of these patients carried the common late-onset mutation c.-32-13T > G. We performed Western blot on 11 patients with <1% GAA activity. All produced GAA protein that was at lower levels and/or was abnormally processed.Discussion
There is no common mutation associated with <1% GAA activity in late-onset Pompe disease patients. Most patients produce unprocessed forms of GAA protein compared with patients with higher GAA activity.Full Text
Duke Authors
Cited Authors
- Bali, DS; Tolun, AA; Goldstein, JL; Dai, J; Kishnani, PS
Published Date
- May 2011
Published In
Volume / Issue
- 43 / 5
Start / End Page
- 665 - 670
PubMed ID
- 21484825
Pubmed Central ID
- 21484825
Electronic International Standard Serial Number (EISSN)
- 1097-4598
International Standard Serial Number (ISSN)
- 0148-639X
Digital Object Identifier (DOI)
- 10.1002/mus.21933
Language
- eng