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Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon.

Publication ,  Journal Article
Schmid, AK; Pan, M; Sharma, K; Baliga, NS
Published in: Nucleic acids research
April 2011

Because iron toxicity and deficiency are equally life threatening, maintaining intracellular iron levels within a narrow optimal range is critical for nearly all known organisms. However, regulatory mechanisms that establish homeostasis are not well understood in organisms that dwell in environments at the extremes of pH, temperature, and salinity. Under conditions of limited iron, the extremophile Halobacterium salinarum, a salt-loving archaeon, mounts a specific response to scavenge iron for growth. We have identified and characterized the role of two transcription factors (TFs), Idr1 and Idr2, in regulating this important response. An integrated systems analysis of TF knockout gene expression profiles and genome-wide binding locations in the presence and absence of iron has revealed that these TFs operate collaboratively to maintain iron homeostasis. In the presence of iron, Idr1 and Idr2 bind near each other at 24 loci in the genome, where they are both required to repress some genes. By contrast, Idr1 and Idr2 are both necessary to activate other genes in a putative a feed forward loop. Even at loci bound independently, the two TFs target different genes with similar functions in iron homeostasis. We discuss conserved and unique features of the Idr1-Idr2 system in the context of similar systems in organisms from other domains of life.

Duke Scholars

Published In

Nucleic acids research

DOI

EISSN

1362-4962

ISSN

0305-1048

Publication Date

April 2011

Volume

39

Issue

7

Start / End Page

2519 / 2533

Related Subject Headings

  • Transcription Factors
  • Sequence Homology, Amino Acid
  • Regulon
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Iron
  • Homeostasis
  • Halobacterium salinarum
  • Gene Expression Regulation, Archaeal
  • Gene Deletion
 

Citation

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Schmid, A. K., Pan, M., Sharma, K., & Baliga, N. S. (2011). Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon. Nucleic Acids Research, 39(7), 2519–2533. https://doi.org/10.1093/nar/gkq1211
Schmid, Amy K., Min Pan, Kriti Sharma, and Nitin S. Baliga. “Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon.Nucleic Acids Research 39, no. 7 (April 2011): 2519–33. https://doi.org/10.1093/nar/gkq1211.
Schmid AK, Pan M, Sharma K, Baliga NS. Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon. Nucleic acids research. 2011 Apr;39(7):2519–33.
Schmid, Amy K., et al. “Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon.Nucleic Acids Research, vol. 39, no. 7, Apr. 2011, pp. 2519–33. Epmc, doi:10.1093/nar/gkq1211.
Schmid AK, Pan M, Sharma K, Baliga NS. Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon. Nucleic acids research. 2011 Apr;39(7):2519–2533.
Journal cover image

Published In

Nucleic acids research

DOI

EISSN

1362-4962

ISSN

0305-1048

Publication Date

April 2011

Volume

39

Issue

7

Start / End Page

2519 / 2533

Related Subject Headings

  • Transcription Factors
  • Sequence Homology, Amino Acid
  • Regulon
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Iron
  • Homeostasis
  • Halobacterium salinarum
  • Gene Expression Regulation, Archaeal
  • Gene Deletion