Skip to main content
Journal cover image

Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.

Publication ,  Journal Article
Mistry, NN; Thomas, A; Kaushik, SS; Johnson, GA; Driehuys, B
Published in: Magn Reson Med
March 2010

The capability to use high-resolution (3)He MRI to depict regional ventilation changes and airway narrowing in mice challenged with methacholine (MCh) offers the opportunity to gain new insights into the study of asthma. However, to fully exploit the value of this novel technique, it is important to move beyond visual inspection of the images toward automated and quantitative analysis. To address this gap, we describe a postprocessing approach to create ventilation difference maps to better visualize and quantify regional ventilation changes before and after MCh challenge. We show that difference maps reveal subtle changes in airway caliber, and highlight both focal and diffuse regional alterations in ventilation. Ventilation changes include both hypoventilation and compensatory areas of hyperventilation. The difference maps can be quantified by a histogram plot of the ventilation changes, in which the standard deviation increases with MCh dose (R(2) = 0.89). This method of analysis is shown to be more sensitive than simple threshold-based detection of gross ventilation defects.

Duke Scholars

Published In

Magn Reson Med

DOI

EISSN

1522-2594

Publication Date

March 2010

Volume

63

Issue

3

Start / End Page

658 / 666

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Reproducibility of Results
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Methacholine Chloride
  • Magnetic Resonance Imaging
  • Isotopes
  • Image Enhancement
  • Helium
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mistry, N. N., Thomas, A., Kaushik, S. S., Johnson, G. A., & Driehuys, B. (2010). Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine. Magn Reson Med, 63(3), 658–666. https://doi.org/10.1002/mrm.22311
Mistry, Nilesh N., Abraham Thomas, S Sivaram Kaushik, G Allan Johnson, and Bastiaan Driehuys. “Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.Magn Reson Med 63, no. 3 (March 2010): 658–66. https://doi.org/10.1002/mrm.22311.
Mistry NN, Thomas A, Kaushik SS, Johnson GA, Driehuys B. Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine. Magn Reson Med. 2010 Mar;63(3):658–66.
Mistry, Nilesh N., et al. “Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.Magn Reson Med, vol. 63, no. 3, Mar. 2010, pp. 658–66. Pubmed, doi:10.1002/mrm.22311.
Mistry NN, Thomas A, Kaushik SS, Johnson GA, Driehuys B. Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine. Magn Reson Med. 2010 Mar;63(3):658–666.
Journal cover image

Published In

Magn Reson Med

DOI

EISSN

1522-2594

Publication Date

March 2010

Volume

63

Issue

3

Start / End Page

658 / 666

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Reproducibility of Results
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Methacholine Chloride
  • Magnetic Resonance Imaging
  • Isotopes
  • Image Enhancement
  • Helium