Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.

Journal Article (Journal Article)

The capability to use high-resolution (3)He MRI to depict regional ventilation changes and airway narrowing in mice challenged with methacholine (MCh) offers the opportunity to gain new insights into the study of asthma. However, to fully exploit the value of this novel technique, it is important to move beyond visual inspection of the images toward automated and quantitative analysis. To address this gap, we describe a postprocessing approach to create ventilation difference maps to better visualize and quantify regional ventilation changes before and after MCh challenge. We show that difference maps reveal subtle changes in airway caliber, and highlight both focal and diffuse regional alterations in ventilation. Ventilation changes include both hypoventilation and compensatory areas of hyperventilation. The difference maps can be quantified by a histogram plot of the ventilation changes, in which the standard deviation increases with MCh dose (R(2) = 0.89). This method of analysis is shown to be more sensitive than simple threshold-based detection of gross ventilation defects.

Full Text

Duke Authors

Cited Authors

  • Mistry, NN; Thomas, A; Kaushik, SS; Johnson, GA; Driehuys, B

Published Date

  • March 2010

Published In

Volume / Issue

  • 63 / 3

Start / End Page

  • 658 - 666

PubMed ID

  • 20187176

Pubmed Central ID

  • PMC2872792

Electronic International Standard Serial Number (EISSN)

  • 1522-2594

Digital Object Identifier (DOI)

  • 10.1002/mrm.22311


  • eng

Conference Location

  • United States