Accentuation of differentially expressed proteins using phage technology.
Journal Article
Protein profiling is frequently used to elucidate disease-specific or differentially expressed proteins. While recent developments have resulted in improved differential profiling, alternative expression platforms that complement existing techniques are continually being explored. We developed a novel method utilizing the amplification and selection capabilities of random peptide-expressing M13 bacteriophage to accentuate differentially expressed proteins in biologic specimens. While the current study used this method to demonstrate differentially expressed proteins in lung cancer tissue in comparison to normal lung tissue, this approach is applicable to a wide range of sample types.
Full Text
Duke Authors
Cited Authors
- Suber, RL; Flanders, VL; Campa, MJ; Patz, EF
Published Date
- October 15, 2004
Published In
Volume / Issue
- 333 / 2
Start / End Page
- 351 - 357
PubMed ID
- 15450812
International Standard Serial Number (ISSN)
- 0003-2697
Digital Object Identifier (DOI)
- 10.1016/j.ab.2004.06.029
Language
- eng
Conference Location
- United States