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Accentuation of differentially expressed proteins using phage technology.

Publication ,  Journal Article
Suber, RL; Flanders, VL; Campa, MJ; Patz, EF
Published in: Anal Biochem
October 15, 2004

Protein profiling is frequently used to elucidate disease-specific or differentially expressed proteins. While recent developments have resulted in improved differential profiling, alternative expression platforms that complement existing techniques are continually being explored. We developed a novel method utilizing the amplification and selection capabilities of random peptide-expressing M13 bacteriophage to accentuate differentially expressed proteins in biologic specimens. While the current study used this method to demonstrate differentially expressed proteins in lung cancer tissue in comparison to normal lung tissue, this approach is applicable to a wide range of sample types.

Duke Scholars

Published In

Anal Biochem

DOI

ISSN

0003-2697

Publication Date

October 15, 2004

Volume

333

Issue

2

Start / End Page

351 / 357

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Proteomics
  • Proteins
  • Lung Neoplasms
  • Lung
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling
  • Cells, Cultured
  • Biochemistry & Molecular Biology
 

Citation

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Suber, R. L., Flanders, V. L., Campa, M. J., & Patz, E. F. (2004). Accentuation of differentially expressed proteins using phage technology. Anal Biochem, 333(2), 351–357. https://doi.org/10.1016/j.ab.2004.06.029
Suber, R Lee, Vincent L. Flanders, Michael J. Campa, and Edward F. Patz. “Accentuation of differentially expressed proteins using phage technology.Anal Biochem 333, no. 2 (October 15, 2004): 351–57. https://doi.org/10.1016/j.ab.2004.06.029.
Suber RL, Flanders VL, Campa MJ, Patz EF. Accentuation of differentially expressed proteins using phage technology. Anal Biochem. 2004 Oct 15;333(2):351–7.
Suber, R. Lee, et al. “Accentuation of differentially expressed proteins using phage technology.Anal Biochem, vol. 333, no. 2, Oct. 2004, pp. 351–57. Pubmed, doi:10.1016/j.ab.2004.06.029.
Suber RL, Flanders VL, Campa MJ, Patz EF. Accentuation of differentially expressed proteins using phage technology. Anal Biochem. 2004 Oct 15;333(2):351–357.
Journal cover image

Published In

Anal Biochem

DOI

ISSN

0003-2697

Publication Date

October 15, 2004

Volume

333

Issue

2

Start / End Page

351 / 357

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Proteomics
  • Proteins
  • Lung Neoplasms
  • Lung
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling
  • Cells, Cultured
  • Biochemistry & Molecular Biology