Genetic dissection of the mouse CNS using magnetic resonance microscopy.

Journal Article (Journal Article;Review)

PURPOSE OF REVIEW: Advances in magnetic resonance microscopy (MRM) make it practical to map gene variants responsible for structural variation in brains of many species, including mice and humans. We review results of a systematic genetic analysis of MRM data using as a case study a family of well characterized lines of mice. RECENT ADVANCES: MRM has matured to the point that we can generate high contrast, high-resolution images even for species as small as a mouse, with a brain merely 1/3000th the size of humans. We generated 21.5-micron data sets for a diverse panel of BXD mouse strains to gauge the extent of genetic variation, and as a prelude to comprehensive genetic and genomic analyses. Here we review MRM capabilities and image segmentation methods; heritability of brain variation; covariation of the sizes of brain regions; and correlations between MRM and classical histological data sets. SUMMARY: The combination of high throughput MRM and genomics will improve our understanding of the genetic basis of structure-function correlations. Sophisticated mouse models will be critical in converting correlations into mechanisms and in determining genetic and epigenetic causes of differences in disease susceptibility.

Full Text

Duke Authors

Cited Authors

  • Badea, A; Johnson, GA; Williams, RW

Published Date

  • August 2009

Published In

Volume / Issue

  • 22 / 4

Start / End Page

  • 379 - 386

PubMed ID

  • 19542887

Pubmed Central ID

  • PMC2734144

Electronic International Standard Serial Number (EISSN)

  • 1473-6551

Digital Object Identifier (DOI)

  • 10.1097/WCO.0b013e32832d9b86


  • eng

Conference Location

  • England