Synthesis of N-succinimidyl 4-[18F]fluorobenzoate, an agent for labeling proteins and peptides with 18F.
Journal Article (Journal Article)
This protocol describes the step-by-step procedure for the synthesis of N-succinimidyl 4-[18F]fluorobenzoate ([18F]SFB), an agent widely used for labeling proteins and peptides with the positron-emitting radionuclide 18F. The protocols for the synthesis of unlabeled SFB and the quaternary salt precursor 4-formyl-N,N,N-trimethyl benzenaminium trifluoromethane sulfonate also are described. For the [18F]SFB synthesis, the quaternary salt is first converted to 4-[18F]fluorobenzaldehyde. Oxidation of the latter provides 4-[18F]fluorobenzoic acid, which is converted to [18F]SFB by treatment with N,N-disuccinimidyl carbonate. Using this method, [18F]SFB can be synthesized in decay-corrected radiochemical yields of 30%-35% and a specific radioactivity of 11-12 GBq micromol(-1). The total synthesis and purification time required is about 80 min, starting from delivery of the [18F]fluoride. [18F]SFB remains an optimal reagent for labeling proteins and peptides with 18F because of good conjugation yields and metabolic stability.
Full Text
Duke Authors
Cited Authors
- Vaidyanathan, G; Zalutsky, MR
Published Date
- 2006
Published In
Volume / Issue
- 1 / 4
Start / End Page
- 1655 - 1661
PubMed ID
- 17487148
Pubmed Central ID
- 17487148
Electronic International Standard Serial Number (EISSN)
- 1750-2799
Digital Object Identifier (DOI)
- 10.1038/nprot.2006.264
Language
- eng
Conference Location
- England