Error correction in gene synthesis technology.


Journal Article (Review)

Accurate, economical and high-throughput gene and genome synthesis is essential to the development of synthetic biology and biotechnology. New large-scale gene synthesis methods harnessing the power of DNA microchips have recently been demonstrated. Yet, the technology is still compromised by a high occurrence of errors in the synthesized products. These errors still require substantial effort to correct. To solve this bottleneck, novel approaches based on new chemistry, enzymology or next generation sequencing have emerged. This review discusses these new trends and promising strategies of error filtration, correction and prevention in de novo gene and genome synthesis. Continued innovation in error correction technologies will enable affordable and large-scale gene and genome synthesis in the near future.

Full Text

Cited Authors

  • Ma, S; Saaem, I; Tian, J

Published Date

  • March 2012

Published In

Volume / Issue

  • 30 / 3

Start / End Page

  • 147 - 154

PubMed ID

  • 22209624

Pubmed Central ID

  • 22209624

Electronic International Standard Serial Number (EISSN)

  • 1879-3096

International Standard Serial Number (ISSN)

  • 0167-7799

Digital Object Identifier (DOI)

  • 10.1016/j.tibtech.2011.10.002


  • eng