A validated chiral HPLC method for the enantiomeric separation of tolterodine tartarate.
Journal Article (Journal Article)
An isocratic chiral HPLC method was developed for the separation of tolterodine tartarate enantiomers. The mobile phase consists of n-hexane and isopropyl alcohol in the ratio of 980:20 (v/v) with 1 ml diethylamine and 0.6 ml trifluoroacetic acid. Chiralcel OD-H (250 mm x 4.6mm) column was used at constant room temperature. Flow rate was kept at 0.5 ml/min. This method is capable of detecting the S-isomer up to 0.1 microg/ml. The method was validated in terms of linearity, precision, limit of detection (LOD) and limit of quantification (LOQ).
Full Text
Duke Authors
Cited Authors
- Kumar, YR; Ramulu, G; Vevakanand, VV; Vaidyanathan, G; Srinivas, K; Kumar, MK; Mukkanti, K; Reddy, MS; Venkatraman, S; Suryanarayana, MV
Published Date
- September 3, 2004
Published In
Volume / Issue
- 35 / 5
Start / End Page
- 1279 - 1285
PubMed ID
- 15336373
International Standard Serial Number (ISSN)
- 0731-7085
Digital Object Identifier (DOI)
- 10.1016/j.jpba.2004.03.026
Language
- eng
Conference Location
- England