Phosphorus 31 magnetic resonance spectroscopy of perifused human placental villi under varying oxygen concentrations.

Journal Article (Journal Article)

OBJECTIVE: Initial phosphorus magnetic resonance spectroscopy observations on the oxygen metabolism of placental villi from normal term pregnancies are described. STUDY DESIGN: Villi were suspended in medium and perifused within a custom-designed 30 mm nuclear magnetic resonance probe in a superconducting vertical nuclear magnetic resonance magnet where pH, temperature, and oxygenation were monitored. RESULTS: Phosphorus resonances were observed from adenosine triphosphate, phosphomonoesters. inorganic phosphate, and phosphodiesters. No phosphocreatine signal was observed. The placental villus tissue responded to an increase in oxygen concentration of the perifusate with a rise in the adenosine triphosphate level and a concomitant decline in the inorganic phosphate and the phosphomonoester signals. CONCLUSION: The changes observed reflect continuing dynamic glycolysis and oxidative phosphorylation. The absence of a phosphocreatine peak suggests that aerobic pathways not driven by creatine kinase are important for placental metabolism. Our system demonstrates dynamic oxygen metabolism in perifused viable placental villus tissue by means of magnetic resonance spectroscopy.

Full Text

Duke Authors

Cited Authors

  • Kay, HH; Hawkins, SR; Wang, Y; Mika, DE; Ribeiro, AA; Spicer, LD

Published Date

  • January 1, 1993

Published In

Volume / Issue

  • 168 / 1 Pt 1

Start / End Page

  • 246 - 252

PubMed ID

  • 8420335

International Standard Serial Number (ISSN)

  • 0002-9378

Digital Object Identifier (DOI)

  • 10.1016/s0002-9378(12)90921-7


  • eng

Conference Location

  • United States