Characterization of a microcarrier cell culture system for 23Na MR spectroscopy studies.

Published

Journal Article

A MR spectroscopy method is described for the simultaneous discrimination and observation of sodium from the three compartments created by an intact cell monolayer. Results are reported for Madin Darby Canine Kidney (MDCK) cells, an epithelial-like continuous cell line, cultured on Cytodex 1 microcarrier beads and perfused with medium containing 6 mM dysprosium (III) tripolyphosphate [Dy(TPP)2(7-)] as shift reagent. The sodium spectrum shows three resonances which are assigned to the shifted intrabead (basolateral) and extrabead (apical) pools and the unshifted intracellular pool. Ouabain inhibition of the Na(+)-K(+)-ATPase cellular pump mechanism was used to demonstrate the sensitivity of the method for monitoring intracellular sodium. The supported MDCK cells in this system remained viable after exposure for 5 h to medium containing Dy(TPP)2(7-) at a concentration of 6 mM, as determined by trypan blue dye exclusion and by comparison of the log growth rate and ability to form domes in subsequent generations of exposed cells vs unexposed controls.

Full Text

Duke Authors

Cited Authors

  • Shedd, SF; Spicer, LD

Published Date

  • October 1991

Published In

Volume / Issue

  • 4 / 5

Start / End Page

  • 246 - 253

PubMed ID

  • 1751347

Pubmed Central ID

  • 1751347

International Standard Serial Number (ISSN)

  • 0952-3480

Language

  • eng

Conference Location

  • England