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Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit.

Publication ,  Journal Article
Kaufman, J; Spicer, LD; Siegel, LM
Published in: Biochemistry
March 23, 1993

The isolated hemeprotein subunit of sulfite reductase (SiR-HP) from Escherichia coli consists of a high spin ferric isobacteriochlorin (siroheme) coupled to a diamagnetic [4Fe-4S]2+ cluster. When supplied with an artificial electron donor, such as methyl viologen cation radical, SiR-HP can catalyze the six electron reductions of sulfite to sulfide and nitrite to ammonia. Thus, the hemeprotein subunit appears to represent the minimal protein structure required for multielectron reductase activity. Proton magnetic resonance spectra are reported for the first time on unligated SiR-HP at 300 MHz in all three redox states. The NMR spectrum of high spin ferric siroheme at pH 6.0 was obtained for the purpose of comparing its spectrum with that of oxidized SiR-HP. On the basis of line widths, T1 measurements, and 1D NOE experiments, preliminary assignments have been made for the oxidized enzyme in solution. The pH profile of oxidized SiR-HP is unusual in that a single resonance shows a 9 ppm shift over a range of only 3 pH units with an apparent pK = 6.7 +/- 0.2. Resonances arising from the beta-CH2 protons of cluster cysteines have been assigned using deuterium substitution for all redox states. One beta-CH2 resonance has been tentatively assigned to the bridging cysteine on the basis of chemical shift, T1, line width, and the presence of NOEs to protons from the siroheme ring. The observed pattern of hyperfine shifts can be used as a probe to measure the degree of coupling between siroheme and cluster in solution. The cluster iron sites of the resting (oxidized) enzyme are found to possess both positive and negative spin density which is in good agreement with Mossbauer results on frozen enzyme. The NMR spectrum of the 1-electron reduced form of SiR-HP is consistent with an intermediate spin (S = 1) siroheme. Intermediate spin Fe(II) hemes have only been previously observed in 4-coordinate model compounds. However, the amount of electron density transferred to the cluster, as measured by the isotropic shift of beta-CH2 resonances, is comparable to that present in the fully oxidized enzyme despite diminution of the total amount of unpaired spin density available. Addition of a second electron to SiR-HP, besides generating a reduced S = 1/2 cluster with both upfield and downfield shifted cysteine resonances, converts siroheme to the high spin (S = 2) ferrous state.(ABSTRACT TRUNCATED AT 400 WORDS)

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

March 23, 1993

Volume

32

Issue

11

Start / End Page

2853 / 2867

Location

United States

Related Subject Headings

  • Thermodynamics
  • Protein Conformation
  • Oxidoreductases Acting on Sulfur Group Donors
  • Mathematics
  • Magnetic Resonance Spectroscopy
  • Macromolecular Substances
  • Kinetics
  • Hydrogen-Ion Concentration
  • Hemeproteins
  • Escherichia coli
 

Citation

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Kaufman, J., Spicer, L. D., & Siegel, L. M. (1993). Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit. Biochemistry, 32(11), 2853–2867. https://doi.org/10.1021/bi00062a017
Kaufman, J., L. D. Spicer, and L. M. Siegel. “Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit.Biochemistry 32, no. 11 (March 23, 1993): 2853–67. https://doi.org/10.1021/bi00062a017.
Kaufman J, Spicer LD, Siegel LM. Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit. Biochemistry. 1993 Mar 23;32(11):2853–67.
Kaufman, J., et al. “Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit.Biochemistry, vol. 32, no. 11, Mar. 1993, pp. 2853–67. Pubmed, doi:10.1021/bi00062a017.
Kaufman J, Spicer LD, Siegel LM. Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit. Biochemistry. 1993 Mar 23;32(11):2853–2867.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

March 23, 1993

Volume

32

Issue

11

Start / End Page

2853 / 2867

Location

United States

Related Subject Headings

  • Thermodynamics
  • Protein Conformation
  • Oxidoreductases Acting on Sulfur Group Donors
  • Mathematics
  • Magnetic Resonance Spectroscopy
  • Macromolecular Substances
  • Kinetics
  • Hydrogen-Ion Concentration
  • Hemeproteins
  • Escherichia coli