A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.

Journal Article (Journal Article)

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

Full Text

Duke Authors

Cited Authors

  • Farmer, BT; Venters, RA; Spicer, LD; Wittekind, MG; Müller, L

Published Date

  • March 1992

Published In

Volume / Issue

  • 2 / 2

Start / End Page

  • 195 - 202

PubMed ID

  • 1422152

International Standard Serial Number (ISSN)

  • 0925-2738

Digital Object Identifier (DOI)

  • 10.1007/BF01875530


  • eng

Conference Location

  • Netherlands