Improved excitation pulse bandwidths using shaped pulses, with application to heteronuclear half filters in macromolecular NMR.

Published

Journal Article

The advantageous use of sinc-shaped pulses in heteronuclear half filters is explored for studying biological macromolecules. The typical square, or hard, pulse used in half-filter pulse sequences for heteronuclear excitation results in suboptimal suppression of unwanted resonances due to incomplete inversion of spins. The novel use of short-duration shaped pulses applied at high power achieves more uniform excitation profiles over the extended frequency ranges often needed for heteronuclear filtering. This approach is used in the development of a double-tuned omega 1, omega 2-double-half-filtered, double-quantum-filtered COSY experiment. The efficiency of this experiment incorporating sinc pulses compares favorably with that obtained with square pulses in a mixture of 13C-labeled and unlabeled amino acids. Sinc-pulse-filtered spectra of the 24 kDa methionine repressor protein dimer MetJ, uniformly 13C-labeled expect at two unlabeled methionine residues, were also obtained to demonstrate the utility of this approach in biomacromolecular studies.

Full Text

Duke Authors

Cited Authors

  • Hyre, DE; Spicer, LD

Published Date

  • July 1995

Published In

Volume / Issue

  • 108 / 1

Start / End Page

  • 12 - 21

PubMed ID

  • 7627432

Pubmed Central ID

  • 7627432

International Standard Serial Number (ISSN)

  • 1064-1866

Digital Object Identifier (DOI)

  • 10.1006/jmrb.1995.1097

Language

  • eng

Conference Location

  • United States