A simple osmium post-fixation paraffin-embedment technique to identify lipid accumulation in fish liver using medaka (Oryziaslatipes) eggs and eleutheroembryos as lipid rich models.

Published

Journal Article

Hepatic lipidosis is a non-specific biomarker of effect from pollution exposure in fish. Fatty liver is often misdiagnosed or overlooked in histological assessments due to the decreasing application of specific fat procedures and stains. For example, ethanol dehydration in standard paraffin processing removes lipids, leaving vacuoles of which the precise nature is unknown. Lipids can be identified using osmium post-fixation in semi-thin resin sections or transmission electron microscopy. However, both are expensive and technically demanding procedures, often not available for routine environmental risk assessment and monitoring programs. The current emphasis to reduce and refine animal toxicity testing, requires refinement of the suite of histopathological techniques currently available to maximize information gained from using fish for toxicity testing and as bio-indicators of environmental quality. This investigation has successfully modified an osmium post-fixation technique to conserve lipids in paraffin-embedded tissues using medaka (Oryzias latipes) eleutheroembryos and eggs (embryos) as lipid rich models.

Full Text

Duke Authors

Cited Authors

  • Mondon, JA; Howitt, J; Tosiano, M; Kwok, KWH; Hinton, DE

Published Date

  • January 2011

Published In

Volume / Issue

  • 63 / 5-12

Start / End Page

  • 86 - 90

PubMed ID

  • 21592532

Pubmed Central ID

  • 21592532

Electronic International Standard Serial Number (EISSN)

  • 1879-3363

International Standard Serial Number (ISSN)

  • 0025-326X

Digital Object Identifier (DOI)

  • 10.1016/j.marpolbul.2011.04.006

Language

  • eng