Use of medaka in toxicity testing.

Journal Article (Journal Article)

Small aquarium fishes are increasingly used as animal models, and one of these, the Japanese Medaka (Oryzias latipes), is frequently utilized for toxicity testing. While these vertebrates have many similarities with their terrestrial counterparts, there are differences that must be considered if these organisms are to be used to their highest potential. Commonly, testing may employ either the developing embryo or adults; both are easy to use and work with. To illustrate the utility and breadth of toxicity testing possible using medaka fish, we present protocols for assessing neurotoxicity in developing embryos, evaluating toxicant effects on sexual phenotype after treatment with endocrine-disrupting chemicals by sexual genotyping, and measuring hepatotoxicity in adult fish after treatment with a model hepatotoxicant. The methods run the gamut from immunohistology through PCR to basic histological techniques.

Full Text

Duke Authors

Cited Authors

  • Padilla, S; Cowden, J; Hinton, DE; Yuen, B; Law, S; Kullman, SW; Johnson, R; Hardman, RC; Flynn, K; Au, DWT

Published Date

  • February 2009

Published In

Volume / Issue

  • Chapter 1 /

Start / End Page

  • Unit1.10 -

PubMed ID

  • 20922755

Pubmed Central ID

  • PMC4300524

Electronic International Standard Serial Number (EISSN)

  • 1934-9262

International Standard Serial Number (ISSN)

  • 1934-9254

Digital Object Identifier (DOI)

  • 10.1002/0471140856.tx0110s39


  • eng