Long-term primary culture of epithelial cells from rainbow trout Oncorhynchus mykiss) liver.
Journal Article (Journal Article)
Long-term primary cultures of epithelial cells from rainbow trout (Oncorhynchus mykiss) liver have been established. Nearly homogenous (> 97%) populations of hepatocytes were placed into primary culture and remained viable and proliferative for at least 70 d. In addition to hepatocytes, proliferative biliary cells persisted in the cultures for at least 30 d. Finally, a third type of epithelial cell, which we have termed a "spindle cell," consistently appeared and proliferated to confluence in these cultures. The confluent cultures of spindle cells were successfully subcultured and passaged. The initial behavior, growth, and optimization of serum and media requirements for these cells is described. All three cell types proliferated as measured by thymidine incorporation, autoradiography, proliferating cellular nuclear antigen analysis, and propidium iodine staining. Further efforts to characterize the cells included western blotting and immunohistochemical staining with antibodies to cytokeratins previously reported in fish liver. From these data, it appears that all three cell populations are epithelial in nature. Furthermore, significant changes in actin organization, often indicative of transformation or pluripotent cells, were observed with increased time in primary culture.
Full Text
Duke Authors
Cited Authors
- Ostrander, GK; Blair, JB; Stark, BA; Marley, GM; Bales, WD; Veltri, RW; Hinton, DE; Okihiro, M; Ortego, LS; Hawkins, WE
Published Date
- May 1995
Published In
Volume / Issue
- 31 / 5
Start / End Page
- 367 - 378
PubMed ID
- 7543343
Pubmed Central ID
- 7543343
Electronic International Standard Serial Number (EISSN)
- 1543-706X
International Standard Serial Number (ISSN)
- 1071-2690
Digital Object Identifier (DOI)
- 10.1007/bf02634286
Language
- eng