Generation and characterization of neurogenin1-GFP transgenic medaka with potential for rapid developmental neurotoxicity screening.

Published

Journal Article

Fish models such as zebrafish and medaka are increasingly used as alternatives to rodents in developmental and toxicological studies. These developmental and toxicological studies can be facilitated by the use of transgenic reporters that permit the real-time, noninvasive observation of the fish. Here we report the construction and characterization of transgenic medaka lines expressing green fluorescent protein (GFP) under the control of the zebrafish neurogenin 1 (ngn1) gene promoter. Neurogenin (ngn1) is a helix-loop-helix transcription factor expressed in proliferating neuronal progenitor cells early in neuronal differentiation and plays a crucial role in directing neurogenesis. GFP expression was detected from 24 h post-fertilization until hatching, in a spatial pattern consistent with the previously reported zebrafish ngn1 expression. Temporal expression of the transgene parallels the expression profile of the endogenous medaka ngn1 transcript. Further, we demonstrate that embryos from the transgenic line permit the non-destructive, real-time screening of ngn1 promoter-directed GFP expression in a 96-well format, enabling higher throughput studies of developmental neurotoxicants. This strain has been deposited with and maintained by the National BioResource Project and is available on request (http://www.shigen.nig.ac.jp/medaka/strainDetailAction.do?quickSearch=true&strainId=5660).

Full Text

Duke Authors

Cited Authors

  • Fan, C-Y; Simmons, SO; Law, SHW; Jensen, K; Cowden, J; Hinton, D; Padilla, S; Ramabhadran, R

Published Date

  • September 2011

Published In

Volume / Issue

  • 105 / 1-2

Start / End Page

  • 127 - 135

PubMed ID

  • 21718657

Pubmed Central ID

  • 21718657

Electronic International Standard Serial Number (EISSN)

  • 1879-1514

International Standard Serial Number (ISSN)

  • 0166-445X

Digital Object Identifier (DOI)

  • 10.1016/j.aquatox.2011.05.017

Language

  • eng