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Effects of donor age on proteasome activity and senescence in trabecular meshwork cells.

Publication ,  Journal Article
Caballero, M; Liton, PB; Challa, P; Epstein, DL; Gonzalez, P
Published in: Biochem Biophys Res Commun
October 22, 2004

The mechanisms involved in the progressive malfunction of the trabecular meshwork (TM) in glaucoma are not yet understood. To study age-related changes in human TM cells, we isolated primary TM cell cultures from young (ages 9, 14, and 25) and old (ages 66, 70, and 73) donors, and compared levels of oxidized proteins, autofluorescence, proteasome function, and markers for cellular senescence. TM cells from old donors showed a 3-fold increase in oxidized proteins and a 7.5-fold decrease of proteasome activity. Loss of proteasome function was not associated with decreased proteasome content but with partial replacement of the proteolytic subunit PSMB5 with the inducible subunit LMP7. Cells from old donors also demonstrated features characteristic of cellular senescence associated with phosphorylation of p38MAPK but only a modest increase in p53. These data suggest that age-related proteasome inhibition and cellular senescence could contribute to the pathophysiological alterations of the TM in glaucoma.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 22, 2004

Volume

323

Issue

3

Start / End Page

1048 / 1054

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Tissue Donors
  • Proteasome Endopeptidase Complex
  • Humans
  • Enzyme Activation
  • Child
  • Cellular Senescence
  • Cells, Cultured
  • Cell Survival
  • Cell Division
 

Citation

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Caballero, M., Liton, P. B., Challa, P., Epstein, D. L., & Gonzalez, P. (2004). Effects of donor age on proteasome activity and senescence in trabecular meshwork cells. Biochem Biophys Res Commun, 323(3), 1048–1054. https://doi.org/10.1016/j.bbrc.2004.08.195
Caballero, Montserrat, Paloma B. Liton, Pratap Challa, David L. Epstein, and Pedro Gonzalez. “Effects of donor age on proteasome activity and senescence in trabecular meshwork cells.Biochem Biophys Res Commun 323, no. 3 (October 22, 2004): 1048–54. https://doi.org/10.1016/j.bbrc.2004.08.195.
Caballero M, Liton PB, Challa P, Epstein DL, Gonzalez P. Effects of donor age on proteasome activity and senescence in trabecular meshwork cells. Biochem Biophys Res Commun. 2004 Oct 22;323(3):1048–54.
Caballero, Montserrat, et al. “Effects of donor age on proteasome activity and senescence in trabecular meshwork cells.Biochem Biophys Res Commun, vol. 323, no. 3, Oct. 2004, pp. 1048–54. Pubmed, doi:10.1016/j.bbrc.2004.08.195.
Caballero M, Liton PB, Challa P, Epstein DL, Gonzalez P. Effects of donor age on proteasome activity and senescence in trabecular meshwork cells. Biochem Biophys Res Commun. 2004 Oct 22;323(3):1048–1054.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 22, 2004

Volume

323

Issue

3

Start / End Page

1048 / 1054

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Tissue Donors
  • Proteasome Endopeptidase Complex
  • Humans
  • Enzyme Activation
  • Child
  • Cellular Senescence
  • Cells, Cultured
  • Cell Survival
  • Cell Division