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Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter.

Publication ,  Journal Article
Liton, PB; Liu, X; Stamer, WD; Challa, P; Epstein, DL; Gonzalez, P
Published in: Invest Ophthalmol Vis Sci
January 2005

PURPOSE: To compare the gene expression profile of trabecular meshwork (TM) and Schlemm's canal (SC) primary cultures and to identify promoters for targeting gene expression to specific cells in the outflow pathway. METHODS: The differential gene expression profile of four human TM and three SC primary cultures was analyzed by gene microarrays (Affymetrix, Santa Clara, CA) and confirmed by quantitative real-time PCR. Based on the results, a recombinant adenovirus was constructed with the expression of the reporter gene LacZ driven by the 5' promoter region of the chitinase 3-like 1 (Ch3L1) gene (AdCh3L1-LacZ). The expression of the Ch3L1 promoter was analyzed in human TM and SC cells and in human perfused anterior segments infected with AdCh3L1-LacZ. RESULTS: gamma-Sarcoglycan, fibulin-2, and collagen XV were identified as the genes more highly expressed in SC than in TM cells. Ch3L1 showed the highest levels of differential expression in TM versus SC cells. Expression analysis of the Ch3L1 promoter demonstrated specific expression in a subset of the TM cells in cell culture and in perfused anterior segments. CONCLUSIONS: Comparative analysis of gene expression between SC and TM primary cultures identified several genes with promoters potentially capable of targeting gene expression to specific cells within the outflow pathway. Results with the Ch3L1 promoter indicated that two different cell subtypes may be present in the TM. This study provides a new potential tool to investigate the role of these different cell types in both normal and pathophysiological function of the outflow pathway, with implications for possible future glaucoma gene therapy.

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Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

January 2005

Volume

46

Issue

1

Start / End Page

183 / 190

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Trabecular Meshwork
  • Sarcoglycans
  • Reverse Transcriptase Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Ophthalmology & Optometry
  • Oligonucleotide Array Sequence Analysis
  • Lectins
  • Humans
  • Glycoproteins
 

Citation

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Liton, P. B., Liu, X., Stamer, W. D., Challa, P., Epstein, D. L., & Gonzalez, P. (2005). Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter. Invest Ophthalmol Vis Sci, 46(1), 183–190. https://doi.org/10.1167/iovs.04-0330
Liton, Paloma B., Xialin Liu, W Daniel Stamer, Pratap Challa, David L. Epstein, and Pedro Gonzalez. “Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter.Invest Ophthalmol Vis Sci 46, no. 1 (January 2005): 183–90. https://doi.org/10.1167/iovs.04-0330.
Liton PB, Liu X, Stamer WD, Challa P, Epstein DL, Gonzalez P. Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter. Invest Ophthalmol Vis Sci. 2005 Jan;46(1):183–90.
Liton, Paloma B., et al. “Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter.Invest Ophthalmol Vis Sci, vol. 46, no. 1, Jan. 2005, pp. 183–90. Pubmed, doi:10.1167/iovs.04-0330.
Liton PB, Liu X, Stamer WD, Challa P, Epstein DL, Gonzalez P. Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter. Invest Ophthalmol Vis Sci. 2005 Jan;46(1):183–190.

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

January 2005

Volume

46

Issue

1

Start / End Page

183 / 190

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Trabecular Meshwork
  • Sarcoglycans
  • Reverse Transcriptase Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Ophthalmology & Optometry
  • Oligonucleotide Array Sequence Analysis
  • Lectins
  • Humans
  • Glycoproteins