Minimal determinants for binding activated G alpha from the structure of a G alpha(i1)-peptide dimer.
Journal Article (Journal Article)
G-proteins cycle between an inactive GDP-bound state and an active GTP-bound state, serving as molecular switches that coordinate cellular signaling. We recently used phage display to identify a series of peptides that bind G alpha subunits in a nucleotide-dependent manner [Johnston, C. A., Willard, F. S., Jezyk, M. R., Fredericks, Z., Bodor, E. T., Jones, M. B., Blaesius, R., Watts, V. J., Harden, T. K., Sondek, J., Ramer, J. K., and Siderovski, D. P. (2005) Structure 13, 1069-1080]. Here we describe the structural features and functions of KB-1753, a peptide that binds selectively to GDP x AlF4(-)- and GTPgammaS-bound states of G alpha(i) subunits. KB-1753 blocks interaction of G alpha(transducin) with its effector, cGMP phosphodiesterase, and inhibits transducin-mediated activation of cGMP degradation. Additionally, KB-1753 interferes with RGS protein binding and resultant GAP activity. A fluorescent KB-1753 variant was found to act as a sensor for activated G alpha in vitro. The crystal structure of KB-1753 bound to G alpha(i1) x GDP x AlF4(-) reveals binding to a conserved hydrophobic groove between switch II and alpha3 helices and, along with supporting biochemical data and previous structural analyses, supports the notion that this is the site of effector interactions for G alpha(i) subunits.
Full Text
Duke Authors
Cited Authors
- Johnston, CA; Lobanova, ES; Shavkunov, AS; Low, J; Ramer, JK; Blaesius, R; Fredericks, Z; Willard, FS; Kuhlman, B; Arshavsky, VY; Siderovski, DP
Published Date
- September 26, 2006
Published In
Volume / Issue
- 45 / 38
Start / End Page
- 11390 - 11400
PubMed ID
- 16981699
Pubmed Central ID
- PMC2597383
International Standard Serial Number (ISSN)
- 0006-2960
Digital Object Identifier (DOI)
- 10.1021/bi0613832
Language
- eng
Conference Location
- United States