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Hydraulic pressure stimulates adenosine 3',5'-cyclic monophosphate accumulation in endothelial cells from Schlemm's canal.

Publication ,  Journal Article
Stamer, WD; Roberts, BC; Epstein, DL
Published in: Invest Ophthalmol Vis Sci
August 1999

PURPOSE: Fluid flow across various endothelia results in a variety of intracellular and extracellular adaptations. In the living eye, aqueous humor flows across the surface of endothelial cells on trabecular meshwork (TM) beams and in the juxtacanalicular tissue and through or between a continuous monolayer of endothelial cells that line Schlemm's canal (SC). This study was undertaken to test the hypothesis that fluid flow induces biochemical changes in the endothelial cells of the outflow pathway that may modify outflow resistance. METHODS: Trabecular meshwork and SC cells isolated from the outflow pathway of human cadaveric eyes were seeded onto porous filters, placed in Ussing-type chambers, and subjected to fluid flow driven by a pressure head of 15 mm Hg on their apical surface. Cell lysates were prepared and analyzed for adenosine 3',5'-cyclic monophosphate (cAMP) accumulation. Barrier function of cell monolayers was examined using transendothelial electrical resistance measurements. RESULTS: Three different SC cell strains in 14 independent experiments responded with at least a threefold increase in cAMP that was both time and pressure dependent. Conversely, flow-treated TM cells failed to respond in six independent experiments in which five different TM cell strains were used. Electrical resistance across cell monolayers positively correlated with cAMP accumulation and was calcium sensitive. CONCLUSIONS: cAMP signaling is affected by pressure differentials across SC cell monolayers and provides evidence for the participation of SC cells in the regulation of aqueous outflow.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

August 1999

Volume

40

Issue

9

Start / End Page

1983 / 1988

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Ophthalmology & Optometry
  • Middle Aged
  • Hydrostatic Pressure
  • Humans
  • Endothelium
  • Cyclic AMP
  • Cells, Cultured
  • Cell Separation
  • Aqueous Humor
 

Citation

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Stamer, W. D., Roberts, B. C., & Epstein, D. L. (1999). Hydraulic pressure stimulates adenosine 3',5'-cyclic monophosphate accumulation in endothelial cells from Schlemm's canal. Invest Ophthalmol Vis Sci, 40(9), 1983–1988.
Stamer, W. D., B. C. Roberts, and D. L. Epstein. “Hydraulic pressure stimulates adenosine 3',5'-cyclic monophosphate accumulation in endothelial cells from Schlemm's canal.Invest Ophthalmol Vis Sci 40, no. 9 (August 1999): 1983–88.
Stamer WD, Roberts BC, Epstein DL. Hydraulic pressure stimulates adenosine 3',5'-cyclic monophosphate accumulation in endothelial cells from Schlemm's canal. Invest Ophthalmol Vis Sci. 1999 Aug;40(9):1983–8.
Stamer, W. D., et al. “Hydraulic pressure stimulates adenosine 3',5'-cyclic monophosphate accumulation in endothelial cells from Schlemm's canal.Invest Ophthalmol Vis Sci, vol. 40, no. 9, Aug. 1999, pp. 1983–88.
Stamer WD, Roberts BC, Epstein DL. Hydraulic pressure stimulates adenosine 3',5'-cyclic monophosphate accumulation in endothelial cells from Schlemm's canal. Invest Ophthalmol Vis Sci. 1999 Aug;40(9):1983–1988.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

August 1999

Volume

40

Issue

9

Start / End Page

1983 / 1988

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Ophthalmology & Optometry
  • Middle Aged
  • Hydrostatic Pressure
  • Humans
  • Endothelium
  • Cyclic AMP
  • Cells, Cultured
  • Cell Separation
  • Aqueous Humor