Functional comparison of RGS9 splice isoforms in a living cell.

Published

Journal Article

Two isoforms of the GTPase-activating protein, regulator of G protein signaling 9 (RGS9), control such fundamental functions as vision and behavior. RGS9-1 regulates phototransduction in rods and cones, and RGS9-2 regulates dopamine and opioid signaling in the basal ganglia. To determine their functional differences in the same intact cell, we replaced RGS9-1 with RGS9-2 in mouse rods. Surprisingly, RGS9-2 not only supported normal photoresponse recovery under moderate light conditions but also outperformed RGS9-1 in bright light. This versatility of RGS9-2 results from its ability to inactivate the G protein, transducin, regardless of its effector interactions, whereas RGS9-1 prefers the G protein-effector complex. Such versatility makes RGS9-2 an isoform advantageous for timely signal inactivation across a wide range of stimulus strengths and may explain its predominant representation throughout the nervous system.

Full Text

Duke Authors

Cited Authors

  • Martemyanov, KA; Krispel, CM; Lishko, PV; Burns, ME; Arshavsky, VY

Published Date

  • December 19, 2008

Published In

Volume / Issue

  • 105 / 52

Start / End Page

  • 20988 - 20993

PubMed ID

  • 19098104

Pubmed Central ID

  • 19098104

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0808941106

Language

  • eng