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The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants.

Publication ,  Journal Article
Achneck, HE; Jamiolkowski, RM; Jantzen, AE; Haseltine, JM; Lane, WO; Huang, JK; Galinat, LJ; Serpe, MJ; Lin, F-H; Li, M; Parikh, A; Ma, L ...
Published in: Biomaterials
January 2011

Implantable and extracorporeal cardiovascular devices are commonly made from titanium (Ti) (e.g. Ti-coated Nitinol stents and mechanical circulatory assist devices). Endothelializing the blood-contacting Ti surfaces of these devices would provide them with an antithrombogenic coating that mimics the native lining of blood vessels and the heart. We evaluated the viability and adherence of peripheral blood-derived porcine endothelial progenitor cells (EPCs), seeded onto thin Ti layers on glass slides under static conditions and after exposure to fluid shear stresses. EPCs attached and grew to confluence on Ti in serum-free medium, without preadsorption of proteins. After attachment to Ti for 15 min, less than 5% of the cells detached at a shear stress of 100 dyne / cm(2). Confluent monolayers of EPCs on smooth Ti surfaces (Rq of 10 nm), exposed to 15 or 100 dyne/cm(2) for 48 h, aligned and elongated in the direction of flow and produced nitric oxide dependent on the level of shear stress. EPC-coated Ti surfaces had dramatically reduced platelet adhesion when compared to uncoated Ti surfaces. These results indicate that peripheral blood-derived EPCs adhere and function normally on Ti surfaces. Therefore EPCs may be used to seed cardiovascular devices prior to implantation to ameliorate platelet activation and thrombus formation.

Duke Scholars

Published In

Biomaterials

DOI

EISSN

1878-5905

Publication Date

January 2011

Volume

32

Issue

1

Start / End Page

10 / 18

Location

Netherlands

Related Subject Headings

  • Titanium
  • Sus scrofa
  • Surface Properties
  • Stress, Mechanical
  • Stem Cells
  • Rheology
  • Platelet Adhesiveness
  • Nitric Oxide
  • Materials Testing
  • Implants, Experimental
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Achneck, H. E., Jamiolkowski, R. M., Jantzen, A. E., Haseltine, J. M., Lane, W. O., Huang, J. K., … Lawson, J. H. (2011). The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants. Biomaterials, 32(1), 10–18. https://doi.org/10.1016/j.biomaterials.2010.08.073
Achneck, Hardean E., Ryan M. Jamiolkowski, Alexandra E. Jantzen, Justin M. Haseltine, Whitney O. Lane, Jessica K. Huang, Lauren J. Galinat, et al. “The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants.Biomaterials 32, no. 1 (January 2011): 10–18. https://doi.org/10.1016/j.biomaterials.2010.08.073.
Achneck HE, Jamiolkowski RM, Jantzen AE, Haseltine JM, Lane WO, Huang JK, et al. The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants. Biomaterials. 2011 Jan;32(1):10–8.
Achneck, Hardean E., et al. “The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants.Biomaterials, vol. 32, no. 1, Jan. 2011, pp. 10–18. Pubmed, doi:10.1016/j.biomaterials.2010.08.073.
Achneck HE, Jamiolkowski RM, Jantzen AE, Haseltine JM, Lane WO, Huang JK, Galinat LJ, Serpe MJ, Lin F-H, Li M, Parikh A, Ma L, Chen T, Sileshi B, Milano CA, Wallace CS, Stabler TV, Allen JD, Truskey GA, Lawson JH. The biocompatibility of titanium cardiovascular devices seeded with autologous blood-derived endothelial progenitor cells: EPC-seeded antithrombotic Ti implants. Biomaterials. 2011 Jan;32(1):10–18.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

Publication Date

January 2011

Volume

32

Issue

1

Start / End Page

10 / 18

Location

Netherlands

Related Subject Headings

  • Titanium
  • Sus scrofa
  • Surface Properties
  • Stress, Mechanical
  • Stem Cells
  • Rheology
  • Platelet Adhesiveness
  • Nitric Oxide
  • Materials Testing
  • Implants, Experimental