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Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats.

Publication ,  Journal Article
Kawaguchi, AT; Reddi, AH; Olson, SA; Yinger, KE; Moehring, HD
Published in: J Orthop Trauma
April 2004

OBJECTIVES: To evaluate the effects of local antibiotics on bone morphogenetic protein-induced new bone formation in vivo. DESIGN: In the research laboratory, inactive collagenous bone matrix was reconstituted with 1 microg of recombinant human bone morphogenetic protein-7 and implanted subcutaneously in the thorax bilaterally in 30 male Long-Evans rats. INTERVENTION: In group A (n = 2), the inactive collagenous bone matrix alone was implanted, bilaterally, and one of these pellets treated with either 500 microg tobramycin in aqueous solution or 3 tobramycin-impregnated polymethyl methacrylate beads. In group B (n = 4), the reconstituted pellets were not treated with tobramycin. In group C (n = 8), 1 reconstituted pellet in each rat was treated with 500 microg tobramycin in aqueous solution. In group D (n = 8), 3 tobramycin beads were placed in contact with 1 of the 2 reconstituted pellets in each rat. In group E (n = 8), 3 tobramycin beads were placed on the dorsal surface of 4 of the rats. All rats were killed on day 11. MAIN OUTCOME MEASUREMENT: Bone formation was evaluated by alkaline phosphatase assay and histology. Tobramycin elution from the beads after day 11 was measured by placing the explanted beads into a phosphate buffer solution to incubate for 24 hours. RESULTS: There was no difference in the alkaline phosphatase activity between the tobramycin treated and untreated implants. Histologic evaluation of the implants revealed areas of robust new bone formation in both the tobramycin treated and untreated implants. CONCLUSIONS: The results by both alkaline phosphatase assay and histologic evaluation in this rat model indicate that there is no inhibition of recombinant human bone morphogenetic protein-7-induced new bone formation by locally applied tobramycin. Recombinant human bone morphogenetic protein-7 is osteoinductive in the presence of locally applied tobramycin. A composite osteogenic device containing both tobramycin and recombinant human bone morphogenetic protein-7 may be developed that can simultaneously induce bone healing and decrease the risk for infection.

Duke Scholars

Published In

J Orthop Trauma

DOI

ISSN

0890-5339

Publication Date

April 2004

Volume

18

Issue

4

Start / End Page

225 / 232

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Tobramycin
  • Rats
  • Osteogenesis
  • Orthopedics
  • Models, Animal
  • Male
  • Growth Substances
  • Drug Incompatibility
  • Drug Antagonism
 

Citation

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Kawaguchi, A. T., Reddi, A. H., Olson, S. A., Yinger, K. E., & Moehring, H. D. (2004). Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats. J Orthop Trauma, 18(4), 225–232. https://doi.org/10.1097/00005131-200404000-00006
Kawaguchi, Alan T., A Hari Reddi, Steven A. Olson, Kent E. Yinger, and H David Moehring. “Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats.J Orthop Trauma 18, no. 4 (April 2004): 225–32. https://doi.org/10.1097/00005131-200404000-00006.
Kawaguchi AT, Reddi AH, Olson SA, Yinger KE, Moehring HD. Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats. J Orthop Trauma. 2004 Apr;18(4):225–32.
Kawaguchi, Alan T., et al. “Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats.J Orthop Trauma, vol. 18, no. 4, Apr. 2004, pp. 225–32. Pubmed, doi:10.1097/00005131-200404000-00006.
Kawaguchi AT, Reddi AH, Olson SA, Yinger KE, Moehring HD. Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats. J Orthop Trauma. 2004 Apr;18(4):225–232.

Published In

J Orthop Trauma

DOI

ISSN

0890-5339

Publication Date

April 2004

Volume

18

Issue

4

Start / End Page

225 / 232

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Tobramycin
  • Rats
  • Osteogenesis
  • Orthopedics
  • Models, Animal
  • Male
  • Growth Substances
  • Drug Incompatibility
  • Drug Antagonism