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Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice.

Publication ,  Journal Article
Seifer, DR; Furman, BD; Guilak, F; Olson, SA; Brooks, SC; Kraus, VB
Published in: Osteoarthritis Cartilage
December 2008

OBJECTIVE: We evaluated three methodologies--a calcium sodium alginate compound (CSAC), polyacrylate beads (PABs), and Whatman paper recovery (WPR)--for the ability to recover synovial fluid (SF) from mouse knees in a manner that facilitated biochemical marker analysis. METHODS: Pilot testing of each of these recovery vehicles was conducted using small volumes of waste human SF. CSAC emerged as the method of choice, and was used to recover and quantify SF from the knees of C57BL/6 mice (n=12), six of which were given left knee articular fractures. SF concentrations of cartilage oligomeric matrix protein (COMP) were measured by enzyme-linked immunosorbent assay. RESULTS: The mean concentration ratio [(COMP(left knee))/(COMP(right knee))] was higher in the mice subjected to articular fracture when compared to the non-fracture mice (P=0.026). The mean total COMP ratio (taking into account the quantitative recovery of SF) best discriminated between fracture and non-fracture knees (P=0.004). CONCLUSIONS: Our results provide the first direct evidence of accelerated joint tissue turnover in a mouse model responding to acute joint injury. These data strongly suggest that mouse SF recovery is feasible and that biomarker analysis of collected SF samples can augment traditional histological analyses in mouse models of arthritis.

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Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

December 2008

Volume

16

Issue

12

Start / End Page

1532 / 1538

Location

England

Related Subject Headings

  • Synovial Fluid
  • Pilot Projects
  • Osteoarthritis
  • Mice
  • Lyases
  • Immunohistochemistry
  • Fractures, Bone
  • Cartilage, Articular
  • Biomarkers
  • Arthritis & Rheumatology
 

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Seifer, D. R., Furman, B. D., Guilak, F., Olson, S. A., Brooks, S. C., & Kraus, V. B. (2008). Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice. Osteoarthritis Cartilage, 16(12), 1532–1538. https://doi.org/10.1016/j.joca.2008.04.013
Seifer, D. R., B. D. Furman, F. Guilak, S. A. Olson, S Carroll Brooks, and V Byers Kraus. “Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice.Osteoarthritis Cartilage 16, no. 12 (December 2008): 1532–38. https://doi.org/10.1016/j.joca.2008.04.013.
Seifer DR, Furman BD, Guilak F, Olson SA, Brooks SC, Kraus VB. Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice. Osteoarthritis Cartilage. 2008 Dec;16(12):1532–8.
Seifer, D. R., et al. “Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice.Osteoarthritis Cartilage, vol. 16, no. 12, Dec. 2008, pp. 1532–38. Pubmed, doi:10.1016/j.joca.2008.04.013.
Seifer DR, Furman BD, Guilak F, Olson SA, Brooks SC, Kraus VB. Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice. Osteoarthritis Cartilage. 2008 Dec;16(12):1532–1538.
Journal cover image

Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

December 2008

Volume

16

Issue

12

Start / End Page

1532 / 1538

Location

England

Related Subject Headings

  • Synovial Fluid
  • Pilot Projects
  • Osteoarthritis
  • Mice
  • Lyases
  • Immunohistochemistry
  • Fractures, Bone
  • Cartilage, Articular
  • Biomarkers
  • Arthritis & Rheumatology