Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma.
Journal Article (Journal Article)
Post-traumatic arthritis is a frequent consequence of articular fracture. The mechanisms leading to its development after such injuries have not been clearly delineated. A potential contributing factor is decreased viability of the articular chondrocytes. The object of this study was to characterise the regional variation in the viability of chondrocytes following joint trauma. A total of 29 osteochondral fragments from traumatic injuries to joints that could not be used in articular reconstruction were analysed for cell viability using the fluorescence live/dead assay and for apoptosis employing the TUNEL assay, and compared with cadaver control fragments. Chondrocyte death and apoptosis were significantly greater along the edge of the fracture and in the superficial zone of the osteochondral fragments. The middle and deep zones demonstrated significantly higher viability of the chondrocytes. These findings indicate the presence of both necrotic and apoptotic chondrocytes after joint injury and may provide further insight into the role of chondrocyte death in post-traumatic arthritis.
Full Text
Duke Authors
Cited Authors
- Hembree, WC; Ward, BD; Furman, BD; Zura, RD; Nichols, LA; Guilak, F; Olson, SA
Published Date
- October 2007
Published In
Volume / Issue
- 89 / 10
Start / End Page
- 1388 - 1395
PubMed ID
- 17957084
International Standard Serial Number (ISSN)
- 0301-620X
Digital Object Identifier (DOI)
- 10.1302/0301-620X.89B10.18907
Language
- eng
Conference Location
- England