Protease-activated quantum dot probes.

Published

Journal Article

We have developed a novel nanoparticulate luminescent probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This construct may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Release of AuNPs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 h of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

Full Text

Duke Authors

Cited Authors

  • Chang, E; Miller, JS; Sun, J; Yu, WW; Colvin, VL; Drezek, R; West, JL

Published Date

  • September 2005

Published In

Volume / Issue

  • 334 / 4

Start / End Page

  • 1317 - 1321

PubMed ID

  • 16039606

Pubmed Central ID

  • 16039606

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2005.07.028

Language

  • eng