PEGDA hydrogels with patterned elasticity: Novel tools for the study of cell response to substrate rigidity.

Published

Journal Article

The ability of cells to migrate in response to mechanical gradients (durotaxis) and differential cell behavior in adhesion, spreading, and proliferation in response to substrate rigidity are key factors both in tissue engineering, in which materials must be selected to provide the appropriate mechanical signals, and in studies of mechanisms of diseases such as cancer and atherosclerosis, in which changes in tissue stiffness may inform cell behavior. Using poly(ethylene glycol) diacrylate hydrogels with varying polymer chain length and photolithographic patterning techniques, we are able to provide substrates with spatially patterned, tunable mechanical properties in both gradients and distinct patterns. The hydrogels can be patterned to produce anisotropic structures and exhibit patterned strain under mechanical loading. These hydrogels may be used to study cell response to substrate rigidity in both two and three dimensions and can also be used as a scaffold in tissue-engineering applications.

Full Text

Duke Authors

Cited Authors

  • Nemir, S; Hayenga, HN; West, JL

Published Date

  • February 2010

Published In

Volume / Issue

  • 105 / 3

Start / End Page

  • 636 - 644

PubMed ID

  • 19816965

Pubmed Central ID

  • 19816965

Electronic International Standard Serial Number (EISSN)

  • 1097-0290

International Standard Serial Number (ISSN)

  • 0006-3592

Digital Object Identifier (DOI)

  • 10.1002/bit.22574

Language

  • eng