Modification of surfaces with cell adhesion peptides alters extracellular matrix deposition.

Published

Journal Article

The goal of the current study was to evaluate matrix protein synthesis by cells cultured on materials that had been modified with cell adhesion ligands. We examined the effects of surface peptide density and of peptides with different affinities on the extracellular matrix production of smooth muscle cells, endothelial cells and fibroblasts. While initial adhesion was greatest on the higher density peptide surfaces, all cell types exhibited decreased matrix production on the more highly adhesive surfaces. Similarly, when different peptides were evaluated, matrix production was the lowest on the most adhesive surface and highest on the least adhesive surface. These results suggest that extracellular matrix synthesis may be regulated, to some extent, by signal transduction initiated by adhesion events. This may pose limitations for use of bioactive materials as tissue engineering scaffolds, as matrix production is an important aspect of tissue formation. However, it may be possible to increase matrix production on highly adhesive surfaces using exogenous factors. TGF-beta was shown to increase matrix production by both smooth muscle cells and endothelial cells.

Full Text

Duke Authors

Cited Authors

  • Mann, BK; Tsai, AT; Scott-Burden, T; West, JL

Published Date

  • December 1999

Published In

Volume / Issue

  • 20 / 23-24

Start / End Page

  • 2281 - 2286

PubMed ID

  • 10614934

Pubmed Central ID

  • 10614934

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/s0142-9612(99)00158-1

Language

  • eng