Rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the United States.

Journal Article

A rickettsia related to but distinct from the spotted fever agent, Rickettsia rickettsii, has been detected in 167 (18.9%) of 884 Rhipicephalus sanguineus taken off dogs in central and northern Mississippi. The organisms could readily be isolated in male meadow voles (Microtus pennsylvanicus), where it produced massive infections in the tissues of tunica vaginalis. It was practically nonpathogenic for male guinea pigs, although inoculation of these animals with infected tunica vaginalis of voles afforded in 30 of 38 instances solid immunity to challenge with virulent R. rickettsii. The Rhipicephalus rickettsia grew well in monolayers of chicken embryo fibroblast, Vero, mouse L, and HeLa cells. Cytopathogenic effects were minimal unless large concentrations of rickettsiae were used as inocula. It also could be established in embryonated hen eggs but only after injection of massive doses of L cell-propagated organisms. Serological tests (complement fixation, microagglutination and/or micro immunofluorescence) indicated that the newly described Rickettsia belongs to the spotted fever group but differs from R. rickettsii, R. akari, and R. conorii. Antigenic differences were also demonstrated by direct fluorescence microscopy as well as by vaccine potency and mouse-toxin neutralization tests.

Full Text

Duke Authors

Cited Authors

  • Burgdorfer, W; Sexton, DJ; Gerloff, RK; Anacker, RL; Philip, RN; Thomas, LA

Published Date

  • July 1, 1975

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 205 - 210

PubMed ID

  • 806533

International Standard Serial Number (ISSN)

  • 0019-9567

Language

  • eng

Conference Location

  • United States