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Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro.

Publication ,  Journal Article
Sexton, DJ; O'Reilly, MW; Friel, AM; Morrison, JJ
Published in: Reprod Biol Endocrinol
December 7, 2004

BACKGROUND: 17 alpha-hydroxyprogesterone caproate (17P) administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non-pregnant state. METHODS: Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. The effects of 17P (1 nmol/L -10 micro mol/L) on spontaneous and agonist-induced (oxytocin 0.5 nmol/L for pregnant, phenylephrine 10 micromol/L for non-pregnant) contractions were measured. Integrals of contractile activity, including the mean maximal inhibition values (MMI) observed at the maximal concentration, were compared with those from simultaneously run control strips. RESULTS: There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. The MMI +/- SEM for spontaneous contractions in pregnant myometrium was 4.9% +/- 7.2 (n = 6; P = 0.309) and for oxytocin-induced contractions was 2.2% +/- 1.3 (n = 6; P = 0.128). For non-pregnant myometrium, the MMI +/- SEM for spontaneous contractions was 8.8% +/- 11.0 (n = 6; P = 0.121) and for phenylephrine induced contractions was -7.9% +/- 6.5 (n = 6; P = 0.966). CONCLUSIONS: The putative benefits of 17P for preterm labor prevention are not achieved, even partially, by a direct utero-relaxant effect. These findings outline the possibility that genomic effects of 17P, achieved over long periods of administration, are required for its reported therapeutic benefits.

Duke Scholars

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Published In

Reprod Biol Endocrinol

DOI

EISSN

1477-7827

Publication Date

December 7, 2004

Volume

2

Start / End Page

80

Location

England

Related Subject Headings

  • Uterine Contraction
  • Pregnancy
  • Phenylephrine
  • Oxytocin
  • Obstetrics & Reproductive Medicine
  • Myometrium
  • Isometric Contraction
  • In Vitro Techniques
  • Hysterectomy
  • Hydroxyprogesterones
 

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Sexton, D. J., O’Reilly, M. W., Friel, A. M., & Morrison, J. J. (2004). Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro. Reprod Biol Endocrinol, 2, 80. https://doi.org/10.1186/1477-7827-2-80
Sexton, Donal J., Michael W. O’Reilly, Anne M. Friel, and John J. Morrison. “Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro.Reprod Biol Endocrinol 2 (December 7, 2004): 80. https://doi.org/10.1186/1477-7827-2-80.
Sexton DJ, O’Reilly MW, Friel AM, Morrison JJ. Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro. Reprod Biol Endocrinol. 2004 Dec 7;2:80.
Sexton, Donal J., et al. “Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro.Reprod Biol Endocrinol, vol. 2, Dec. 2004, p. 80. Pubmed, doi:10.1186/1477-7827-2-80.
Sexton DJ, O’Reilly MW, Friel AM, Morrison JJ. Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro. Reprod Biol Endocrinol. 2004 Dec 7;2:80.
Journal cover image

Published In

Reprod Biol Endocrinol

DOI

EISSN

1477-7827

Publication Date

December 7, 2004

Volume

2

Start / End Page

80

Location

England

Related Subject Headings

  • Uterine Contraction
  • Pregnancy
  • Phenylephrine
  • Oxytocin
  • Obstetrics & Reproductive Medicine
  • Myometrium
  • Isometric Contraction
  • In Vitro Techniques
  • Hysterectomy
  • Hydroxyprogesterones