High-throughput affinity ranking of antibodies using surface plasmon resonance microarrays.

Journal Article (Journal Article)

A method was developed to rapidly identify high-affinity human antibodies from phage display library selection outputs. It combines high-throughput Fab fragment expression and purification with surface plasmon resonance (SPR) microarrays to determine kinetic constants (kon and koff) for 96 different Fab fragments in a single experiment. Fabs against human tissue kallikrein 1 (hK1, KLK1 gene product) were discovered by phage display, expressed in Escherichia coli in batches of 96, and purified using protein A PhyTip columns. Kinetic constants were obtained for 191 unique anti-hK1 Fabs using the Flexchip SPR microarray device. The highest affinity Fabs discovered had dissociation constants of less than 1 nM. The described SPR method was also used to categorize Fabs according to their ability to recognize an apparent active site epitope. The ability to rapidly determine the affinities of hundreds of antibodies significantly accelerates the discovery of high-affinity antibody leads.

Full Text

Duke Authors

Cited Authors

  • Wassaf, D; Kuang, G; Kopacz, K; Wu, Q-L; Nguyen, Q; Toews, M; Cosic, J; Jacques, J; Wiltshire, S; Lambert, J; Pazmany, CC; Hogan, S; Ladner, RC; Nixon, AE; Sexton, DJ

Published Date

  • April 15, 2006

Published In

Volume / Issue

  • 351 / 2

Start / End Page

  • 241 - 253

PubMed ID

  • 16510109

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2006.01.043


  • eng

Conference Location

  • United States