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Visible light photochemical release of nitric oxide from S-nitrosoglutathione: potential photochemotherapeutic applications.

Publication ,  Journal Article
Sexton, DJ; Muruganandam, A; McKenney, DJ; Mutus, B
Published in: Photochem Photobiol
April 1994

Some aspects of the physiological role of NO may be mediated by stable NO-carriers such as S-nitrosoglutathione and related S-nitrosothiols. In this report we show that irradiation of S-nitrosoglutathione at either absorption band (lambda max = 340 nm or 545 nm) results in the release of nitric oxide. Photolysis of S-nitrosoglutathione at 545 nm exhibited a quantum yield of 0.056 +/- 0.002 and was best approximated by a first-order process with kobs = 4.9 x 10(-7) +/- 0.3 x 10(-7) s-1. The photolytic release of NO from S-nitrosoglutathione resulted in an enhanced cytotoxic effect of S-nitrosoglutathione on HL-60 leukemia cells. That the cytotoxic effect of S-nitrosoglutathione was diminished by the addition of oxyhemoglobin strongly suggests that NO is the cytotoxic species. The finding that NO can be readily liberated from S-nitrosoglutathione by visible radiation indicates that the photochemical properties of this compound in the visible spectrum must be considered in order to obtain meaningful data as to its physiological role and the S-nitrosoglutathione and related compounds may find use as photochemotherapeutic agents.

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Published In

Photochem Photobiol

DOI

ISSN

0031-8655

Publication Date

April 1994

Volume

59

Issue

4

Start / End Page

463 / 467

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • S-Nitrosoglutathione
  • Photolysis
  • Nitroso Compounds
  • Nitric Oxide
  • Light
  • Leukemia, Experimental
  • Humans
  • Glutathione
  • Biophysics
 

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Sexton, D. J., Muruganandam, A., McKenney, D. J., & Mutus, B. (1994). Visible light photochemical release of nitric oxide from S-nitrosoglutathione: potential photochemotherapeutic applications. Photochem Photobiol, 59(4), 463–467. https://doi.org/10.1111/j.1751-1097.1994.tb05065.x
Sexton, D. J., A. Muruganandam, D. J. McKenney, and B. Mutus. “Visible light photochemical release of nitric oxide from S-nitrosoglutathione: potential photochemotherapeutic applications.Photochem Photobiol 59, no. 4 (April 1994): 463–67. https://doi.org/10.1111/j.1751-1097.1994.tb05065.x.
Sexton DJ, Muruganandam A, McKenney DJ, Mutus B. Visible light photochemical release of nitric oxide from S-nitrosoglutathione: potential photochemotherapeutic applications. Photochem Photobiol. 1994 Apr;59(4):463–7.
Sexton, D. J., et al. “Visible light photochemical release of nitric oxide from S-nitrosoglutathione: potential photochemotherapeutic applications.Photochem Photobiol, vol. 59, no. 4, Apr. 1994, pp. 463–67. Pubmed, doi:10.1111/j.1751-1097.1994.tb05065.x.
Sexton DJ, Muruganandam A, McKenney DJ, Mutus B. Visible light photochemical release of nitric oxide from S-nitrosoglutathione: potential photochemotherapeutic applications. Photochem Photobiol. 1994 Apr;59(4):463–467.
Journal cover image

Published In

Photochem Photobiol

DOI

ISSN

0031-8655

Publication Date

April 1994

Volume

59

Issue

4

Start / End Page

463 / 467

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • S-Nitrosoglutathione
  • Photolysis
  • Nitroso Compounds
  • Nitric Oxide
  • Light
  • Leukemia, Experimental
  • Humans
  • Glutathione
  • Biophysics