Platelet glutathione transport: characteristics and evidence for regulation by intraplatelet thiol status.

Journal Article

The present study demonstrates the carrier-mediated uptake of intact glutathione (GSH) by human platelets. Platelet GSH uptake was characterized as being Na+ independent and saturable. The KM, apparent and Vmax, apparent for GSH uptake in platelet plasma membrane vesicles were 28.0 +/- 8.4 microM and 263.5 +/- 28.5 pmol/min per mg protein, respectively. The transport was inhibited by GSH analogs and enhanced by KCl-induced membrane depolarization. GSH transport may be regulated by the intracellular thiol status, since the depletion of intraplatelet GSH with 100 microM 1-chloro-2,4-dinitrobenzene (CDNB) increased GSH uptake by approximately 40%. The KM, apparent and Vmax, apparent for GSH uptake in intact platelets changed from 99.5 +/- 15 microM and 42 +/- 7.5 pmol/min per 10(9) platelets, respectively, to 33.7 +/- 6.7 microM and 21.5 +/- 6.9 pmol/min per 10(9) platelets, respectively, on reducing intraplatelet GSH with 100 microM CDNB.

Full Text

Duke Authors

Cited Authors

  • Sexton, DJ; Mutus, B

Published Date

  • March 1995

Published In

Volume / Issue

  • 73 / 3-4

Start / End Page

  • 155 - 162

PubMed ID

  • 7576489

International Standard Serial Number (ISSN)

  • 0829-8211

Language

  • eng

Conference Location

  • Canada