Sample preparation for MALDI mass spectrometry using an elastomeric device reversibly sealed on the MALDI target.

Published

Journal Article

A new method for improving low-concentration sample recovery and reducing sample preparation steps in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is presented. In the conventional approach, samples are typically desalted and/or concentrated with various techniques and deposited on the MALDI target as small droplets. In this work, we describe a new approach in which an elastomeric device is reversibly sealed on the MALDI target to form a multi-well plate with the MALDI target as the base of the plate. The new format allows a larger volume (5-200 microL) of samples to be deposited on each spot and a series of sample handling processes, including desalting and concentrating, to be performed directly on the MALDI target. Several advantages have been observed: (i) multiple sample transferring steps are avoided; (ii) recovery of low-concentration peptides during sample preparation is improved using a novel desalting method that utilizes the hydrophobic surface of the elastomeric device; and (iii) sequence coverage of the peptide mass fingerprinting map is improved using a novel method in which proteins are immobilized on the hydrophobic surface of the elastomeric device for in-well trypsin digestion, followed by desalting and concentrating the digestion products in the same well.

Full Text

Duke Authors

Cited Authors

  • Chen, X; Murawski, A; Kuang, G; Sexton, DJ; Galbraith, W

Published Date

  • September 1, 2006

Published In

Volume / Issue

  • 78 / 17

Start / End Page

  • 6160 - 6168

PubMed ID

  • 16944898

Pubmed Central ID

  • 16944898

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac060286b

Language

  • eng

Conference Location

  • United States