Assembly and disassembly of DNA polymerase holoenzyme.

Journal Article (Journal Article;Review)

The complex task of genomic replication requires a large collection of proteins properly assembled within the close confines of the replication fork. The mechanism and dynamics of holoenzyme assembly and disassembly have been investigated using steady state and pre-steady state methods as opposed to structural studies, primarily due to the intrinsic transient nature of these protein complexes during DNA replication. The key step in bacteriophage T4 holoenzyme assembly involves ATP hydrolysis, whereas disassembly is mediated by subunit dissociation of the clamp protein in an ATP-independent manner.

Full Text

Duke Authors

Cited Authors

  • Sexton, DJ; Berdis, AJ; Benkovic, SJ

Published Date

  • October 1997

Published In

Volume / Issue

  • 1 / 3

Start / End Page

  • 316 - 322

PubMed ID

  • 9667868

Pubmed Central ID

  • 9667868

International Standard Serial Number (ISSN)

  • 1367-5931

Digital Object Identifier (DOI)

  • 10.1016/s1367-5931(97)80068-3


  • eng

Conference Location

  • England