High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.

Journal Article (Journal Article)

We present a method for high-resolution reconstruction of fluorescent images of the mouse thorax. It features an anatomically guided sampling method to retrospectively eliminate problematic data and a parallel Monte Carlo software package to compute the Jacobian matrix for the inverse problem. The proposed method was capable of resolving microliter-sized femtomole amount of quantum dot inclusions closely located in the middle of the mouse thorax. The reconstruction was verified against co-registered micro-CT data. Using the proposed method, the new system achieved significantly higher resolution and sensitivity compared to our previous system consisting of the same hardware. This method can be applied to any system utilizing similar imaging principles to improve imaging performance.

Full Text

Duke Authors

Cited Authors

  • Zhang, X; Badea, C; Hood, G; Wetzel, A; Qi, Y; Stiles, J; Johnson, GA

Published Date

  • September 1, 2011

Published In

Volume / Issue

  • 2 / 9

Start / End Page

  • 2449 - 2460

PubMed ID

  • 21991539

Pubmed Central ID

  • PMC3184855

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.2.002449


  • eng

Conference Location

  • United States