High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.
Journal Article (Journal Article)
We present a method for high-resolution reconstruction of fluorescent images of the mouse thorax. It features an anatomically guided sampling method to retrospectively eliminate problematic data and a parallel Monte Carlo software package to compute the Jacobian matrix for the inverse problem. The proposed method was capable of resolving microliter-sized femtomole amount of quantum dot inclusions closely located in the middle of the mouse thorax. The reconstruction was verified against co-registered micro-CT data. Using the proposed method, the new system achieved significantly higher resolution and sensitivity compared to our previous system consisting of the same hardware. This method can be applied to any system utilizing similar imaging principles to improve imaging performance.
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Duke Authors
Cited Authors
- Zhang, X; Badea, C; Hood, G; Wetzel, A; Qi, Y; Stiles, J; Johnson, GA
Published Date
- September 1, 2011
Published In
Volume / Issue
- 2 / 9
Start / End Page
- 2449 - 2460
PubMed ID
- 21991539
Pubmed Central ID
- PMC3184855
Electronic International Standard Serial Number (EISSN)
- 2156-7085
Digital Object Identifier (DOI)
- 10.1364/BOE.2.002449
Language
- eng
Conference Location
- United States