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High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.

Publication ,  Journal Article
Zhang, X; Badea, C; Hood, G; Wetzel, A; Qi, Y; Stiles, J; Johnson, GA
Published in: Biomed Opt Express
September 1, 2011

We present a method for high-resolution reconstruction of fluorescent images of the mouse thorax. It features an anatomically guided sampling method to retrospectively eliminate problematic data and a parallel Monte Carlo software package to compute the Jacobian matrix for the inverse problem. The proposed method was capable of resolving microliter-sized femtomole amount of quantum dot inclusions closely located in the middle of the mouse thorax. The reconstruction was verified against co-registered micro-CT data. Using the proposed method, the new system achieved significantly higher resolution and sensitivity compared to our previous system consisting of the same hardware. This method can be applied to any system utilizing similar imaging principles to improve imaging performance.

Duke Scholars

Published In

Biomed Opt Express

DOI

EISSN

2156-7085

Publication Date

September 1, 2011

Volume

2

Issue

9

Start / End Page

2449 / 2460

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

Citation

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ICMJE
MLA
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Zhang, X., Badea, C., Hood, G., Wetzel, A., Qi, Y., Stiles, J., & Johnson, G. A. (2011). High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing. Biomed Opt Express, 2(9), 2449–2460. https://doi.org/10.1364/BOE.2.002449
Zhang, Xiaofeng, Cristian Badea, Greg Hood, Arthur Wetzel, Yi Qi, Joel Stiles, and G Allan Johnson. “High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.Biomed Opt Express 2, no. 9 (September 1, 2011): 2449–60. https://doi.org/10.1364/BOE.2.002449.
Zhang X, Badea C, Hood G, Wetzel A, Qi Y, Stiles J, et al. High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing. Biomed Opt Express. 2011 Sep 1;2(9):2449–60.
Zhang, Xiaofeng, et al. “High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.Biomed Opt Express, vol. 2, no. 9, Sept. 2011, pp. 2449–60. Pubmed, doi:10.1364/BOE.2.002449.
Zhang X, Badea C, Hood G, Wetzel A, Qi Y, Stiles J, Johnson GA. High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing. Biomed Opt Express. 2011 Sep 1;2(9):2449–2460.
Journal cover image

Published In

Biomed Opt Express

DOI

EISSN

2156-7085

Publication Date

September 1, 2011

Volume

2

Issue

9

Start / End Page

2449 / 2460

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics