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Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model.

Publication ,  Journal Article
Stroncek, JD; Ren, LC; Klitzman, B; Reichert, WM
Published in: Acta Biomater
January 2012

Late outgrowth endothelial progenitor cells (EPCs) derived from the peripheral blood of patients with significant coronary artery disease were sodded into the lumens of small diameter expanded polytetrafluoroethylene (ePTFE) vascular grafts. Grafts (1mm inner diameter) were denucleated and sodded either with native EPCs or with EPCs transfected with an adenoviral vector containing the gene for human thrombomodulin (EPC+AdTM). EPC+AdTM was shown to increase the in vitro rate of graft activated protein C (APC) production 4-fold over grafts sodded with untransfected EPCs (p<0.05). Unsodded control and EPC-sodded and EPC+AdTM-sodded grafts were implanted bilaterally into the femoral arteries of athymic rats for 7 or 28 days. Unsodded control grafts, both with and without denucleation treatment, each exhibited 7 day patency rates of 25%. Unsodded grafts showed extensive thrombosis and were not tested for patency over 28 days. In contrast, grafts sodded with untransfected EPCs or EPC+AdTM both had 7 day patency rates of 88-89% and 28 day patency rates of 75-88%. Intimal hyperplasia was observed near both the proximal and distal anastomoses in all sodded graft conditions but did not appear to be the primary occlusive failure event. This in vivo study suggests autologous EPCs derived from the peripheral blood of patients with coronary artery disease may improve the performance of synthetic vascular grafts, although no differences were observed between untransfected EPCs and TM transfected EPCs.

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Published In

Acta Biomater

DOI

EISSN

1878-7568

Publication Date

January 2012

Volume

8

Issue

1

Start / End Page

201 / 208

Location

England

Related Subject Headings

  • Vascular Patency
  • Vascular Grafting
  • Tunica Intima
  • Stem Cells
  • Rats, Nude
  • Rats
  • Protein C
  • Male
  • Hyperplasia
  • Humans
 

Citation

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ICMJE
MLA
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Stroncek, J. D., Ren, L. C., Klitzman, B., & Reichert, W. M. (2012). Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model. Acta Biomater, 8(1), 201–208. https://doi.org/10.1016/j.actbio.2011.09.002
Stroncek, J. D., L. C. Ren, B. Klitzman, and W. M. Reichert. “Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model.Acta Biomater 8, no. 1 (January 2012): 201–8. https://doi.org/10.1016/j.actbio.2011.09.002.
Stroncek JD, Ren LC, Klitzman B, Reichert WM. Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model. Acta Biomater. 2012 Jan;8(1):201–8.
Stroncek, J. D., et al. “Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model.Acta Biomater, vol. 8, no. 1, Jan. 2012, pp. 201–08. Pubmed, doi:10.1016/j.actbio.2011.09.002.
Stroncek JD, Ren LC, Klitzman B, Reichert WM. Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model. Acta Biomater. 2012 Jan;8(1):201–208.
Journal cover image

Published In

Acta Biomater

DOI

EISSN

1878-7568

Publication Date

January 2012

Volume

8

Issue

1

Start / End Page

201 / 208

Location

England

Related Subject Headings

  • Vascular Patency
  • Vascular Grafting
  • Tunica Intima
  • Stem Cells
  • Rats, Nude
  • Rats
  • Protein C
  • Male
  • Hyperplasia
  • Humans