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A liposomal nanoscale contrast agent for preclinical CT in mice.

Publication ,  Journal Article
Mukundan, S; Ghaghada, KB; Badea, CT; Kao, C-Y; Hedlund, LW; Provenzale, JM; Johnson, GA; Chen, E; Bellamkonda, RV; Annapragada, A
Published in: AJR Am J Roentgenol
February 2006

OBJECTIVE: The goal of this study was to determine if an iodinated, liposomal contrast agent could be used for high-resolution, micro-CT of low-contrast, small-size vessels in a murine model. MATERIALS AND METHODS: A second-generation, liposomal blood pool contrast agent encapsulating a high concentration of iodine (83-105 mg I/mL) was evaluated. A total of five mice weighing between 20 and 28 g were infused with equivalent volume doses (500 microL of contrast agent/25 g of mouse weight) and imaged with our micro-CT system for intervals of up to 240 min postinfusion. The animals were anesthetized, mechanically ventilated, and vital signs monitored allowing for simultaneous cardiac and respiratory gating of image acquisition. RESULTS: Initial enhancement of about 900 H in the aorta was obtained, which decreased to a plateau level of approximately 800 H after 2 hr. Excellent contrast discrimination was shown between the myocardium and cardiac blood pool (650-700 H). No significant nephrogram was identified, indicating the absence of renal clearance of the agent. CONCLUSION: The liposomal-based iodinated contrast agent shows long residence time in the blood pool, very high attenuation within submillimeter vessels, and no significant renal clearance rendering it an effective contrast agent for murine vascular imaging using a micro-CT scanner.

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Published In

AJR Am J Roentgenol

DOI

ISSN

0361-803X

Publication Date

February 2006

Volume

186

Issue

2

Start / End Page

300 / 307

Location

United States

Related Subject Headings

  • Triiodobenzoic Acids
  • Tomography, X-Ray Computed
  • Radiography, Thoracic
  • Nuclear Medicine & Medical Imaging
  • Mice
  • Liposomes
  • Image Processing, Computer-Assisted
  • Contrast Media
  • Animals
  • 3202 Clinical sciences
 

Citation

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Mukundan, S., Ghaghada, K. B., Badea, C. T., Kao, C.-Y., Hedlund, L. W., Provenzale, J. M., … Annapragada, A. (2006). A liposomal nanoscale contrast agent for preclinical CT in mice. AJR Am J Roentgenol, 186(2), 300–307. https://doi.org/10.2214/AJR.05.0523
Mukundan, Srinivasan, Ketan B. Ghaghada, Cristian T. Badea, Chen-Yu Kao, Laurence W. Hedlund, James M. Provenzale, G Allan Johnson, Emmanuel Chen, Ravi V. Bellamkonda, and Ananth Annapragada. “A liposomal nanoscale contrast agent for preclinical CT in mice.AJR Am J Roentgenol 186, no. 2 (February 2006): 300–307. https://doi.org/10.2214/AJR.05.0523.
Mukundan S, Ghaghada KB, Badea CT, Kao C-Y, Hedlund LW, Provenzale JM, et al. A liposomal nanoscale contrast agent for preclinical CT in mice. AJR Am J Roentgenol. 2006 Feb;186(2):300–7.
Mukundan, Srinivasan, et al. “A liposomal nanoscale contrast agent for preclinical CT in mice.AJR Am J Roentgenol, vol. 186, no. 2, Feb. 2006, pp. 300–07. Pubmed, doi:10.2214/AJR.05.0523.
Mukundan S, Ghaghada KB, Badea CT, Kao C-Y, Hedlund LW, Provenzale JM, Johnson GA, Chen E, Bellamkonda RV, Annapragada A. A liposomal nanoscale contrast agent for preclinical CT in mice. AJR Am J Roentgenol. 2006 Feb;186(2):300–307.

Published In

AJR Am J Roentgenol

DOI

ISSN

0361-803X

Publication Date

February 2006

Volume

186

Issue

2

Start / End Page

300 / 307

Location

United States

Related Subject Headings

  • Triiodobenzoic Acids
  • Tomography, X-Ray Computed
  • Radiography, Thoracic
  • Nuclear Medicine & Medical Imaging
  • Mice
  • Liposomes
  • Image Processing, Computer-Assisted
  • Contrast Media
  • Animals
  • 3202 Clinical sciences