A liposomal nanoscale contrast agent for preclinical CT in mice.

Published

Journal Article

OBJECTIVE: The goal of this study was to determine if an iodinated, liposomal contrast agent could be used for high-resolution, micro-CT of low-contrast, small-size vessels in a murine model. MATERIALS AND METHODS: A second-generation, liposomal blood pool contrast agent encapsulating a high concentration of iodine (83-105 mg I/mL) was evaluated. A total of five mice weighing between 20 and 28 g were infused with equivalent volume doses (500 microL of contrast agent/25 g of mouse weight) and imaged with our micro-CT system for intervals of up to 240 min postinfusion. The animals were anesthetized, mechanically ventilated, and vital signs monitored allowing for simultaneous cardiac and respiratory gating of image acquisition. RESULTS: Initial enhancement of about 900 H in the aorta was obtained, which decreased to a plateau level of approximately 800 H after 2 hr. Excellent contrast discrimination was shown between the myocardium and cardiac blood pool (650-700 H). No significant nephrogram was identified, indicating the absence of renal clearance of the agent. CONCLUSION: The liposomal-based iodinated contrast agent shows long residence time in the blood pool, very high attenuation within submillimeter vessels, and no significant renal clearance rendering it an effective contrast agent for murine vascular imaging using a micro-CT scanner.

Full Text

Duke Authors

Cited Authors

  • Mukundan, S; Ghaghada, KB; Badea, CT; Kao, C-Y; Hedlund, LW; Provenzale, JM; Johnson, GA; Chen, E; Bellamkonda, RV; Annapragada, A

Published Date

  • February 2006

Published In

Volume / Issue

  • 186 / 2

Start / End Page

  • 300 - 307

PubMed ID

  • 16423931

Pubmed Central ID

  • 16423931

International Standard Serial Number (ISSN)

  • 0361-803X

Digital Object Identifier (DOI)

  • 10.2214/AJR.05.0523

Language

  • eng

Conference Location

  • United States