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Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents.

Publication ,  Journal Article
Maï, W; Badea, CT; Wheeler, CT; Hedlund, LW; Johnson, GA
Published in: Magn Reson Med
April 2005

One can acquire high-resolution pulmonary and cardiac images in live rodents with MR microscopy by synchronizing the image acquisition to the breathing cycle across multiple breaths, and gating to the cardiac cycle. The precision with which one can synchronize image acquisition to the motion defines the ultimate resolution limit that can be attained in such studies. The present work was performed to evaluate how reliably the pulmonary and cardiac structures return to the same position from breath to breath and beat to beat across the prolonged period required for MR microscopy. Radiopaque beads were surgically glued to the abdominal surface of the diaphragm and on the cardiac ventricles of anesthetized, mechanically ventilated rats. We evaluated the range of motion for the beads (relative to a reference vertebral bead) using digital microradiography with two specific biological gating methods: 1) ventilation synchronous acquisition, and 2) both ventilation synchronous and cardiac-gated acquisitions. The standard deviation (SD) of the displacement was < or =100 microm, which is comparable to the resolution limit for in vivo MRI imposed by signal-to-noise ratio (SNR) constraints. With careful control of motion, its impact on resolution can be limited. This work provides the first quantitative measure of the motion-imposed resolution limits for in vivo imaging.

Duke Scholars

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

April 2005

Volume

53

Issue

4

Start / End Page

858 / 865

Location

United States

Related Subject Headings

  • Tungsten
  • Reproducibility of Results
  • Rats, Inbred F344
  • Rats
  • Radiography
  • Nuclear Medicine & Medical Imaging
  • Myocardial Contraction
  • Male
  • Magnetic Resonance Imaging
  • Diaphragm
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Maï, W., Badea, C. T., Wheeler, C. T., Hedlund, L. W., & Johnson, G. A. (2005). Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents. Magn Reson Med, 53(4), 858–865. https://doi.org/10.1002/mrm.20400
Maï, Wilfried, Cristian T. Badea, Charles T. Wheeler, Laurence W. Hedlund, and G Allan Johnson. “Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents.Magn Reson Med 53, no. 4 (April 2005): 858–65. https://doi.org/10.1002/mrm.20400.
Maï W, Badea CT, Wheeler CT, Hedlund LW, Johnson GA. Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents. Magn Reson Med. 2005 Apr;53(4):858–65.
Maï, Wilfried, et al. “Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents.Magn Reson Med, vol. 53, no. 4, Apr. 2005, pp. 858–65. Pubmed, doi:10.1002/mrm.20400.
Maï W, Badea CT, Wheeler CT, Hedlund LW, Johnson GA. Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents. Magn Reson Med. 2005 Apr;53(4):858–865.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

April 2005

Volume

53

Issue

4

Start / End Page

858 / 865

Location

United States

Related Subject Headings

  • Tungsten
  • Reproducibility of Results
  • Rats, Inbred F344
  • Rats
  • Radiography
  • Nuclear Medicine & Medical Imaging
  • Myocardial Contraction
  • Male
  • Magnetic Resonance Imaging
  • Diaphragm