E protein transcription factors are required for the development of CD4(+) lineage T cells.

Published

Journal Article

The double-positive (DP) to single-positive (SP) transition during T cell development is initiated by downregulation of the E protein transcription factors HEB and E2A. Here, we have demonstrated that in addition to regulating the onset of this transition, HEB and E2A also play a separate role in CD4(+) lineage choice. Deletion of HEB and E2A in DP thymocytes specifically blocked the development of CD4(+) lineage T cells. Furthermore, deletion of the E protein inhibitors Id2 and Id3 allowed CD4(+) T cell development but blocked CD8(+) lineage development. Analysis of the CD4(+) lineage transcriptional regulators ThPOK and Gata3 placed HEB and E2A upstream of CD4(+) lineage specification. These studies identify an important role for E proteins in the activation of CD4(+) lineage differentiation as thymocytes undergo the DP to SP transition.

Full Text

Duke Authors

Cited Authors

  • Jones-Mason, ME; Zhao, X; Kappes, D; Lasorella, A; Iavarone, A; Zhuang, Y

Published Date

  • March 23, 2012

Published In

Volume / Issue

  • 36 / 3

Start / End Page

  • 348 - 361

PubMed ID

  • 22425249

Pubmed Central ID

  • 22425249

Electronic International Standard Serial Number (EISSN)

  • 1097-4180

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2012.02.010

Language

  • eng

Conference Location

  • United States