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Disruption of lysosome function promotes tumor growth and metastasis in Drosophila.

Publication ,  Journal Article
Chi, C; Zhu, H; Han, M; Zhuang, Y; Wu, X; Xu, T
Published in: J Biol Chem
July 9, 2010

Lysosome function is essential to many physiological processes. It has been suggested that deregulation of lysosome function could contribute to cancer. Through a genetic screen in Drosophila, we have discovered that mutations disrupting lysosomal degradation pathway components contribute to tumor development and progression. Loss-of-function mutations in the Class C vacuolar protein sorting (VPS) gene, deep orange (dor), dramatically promote tumor overgrowth and invasion of the Ras(V12) cells. Knocking down either of the two other components of the Class C VPS complex, carnation (car) and vps16A, also renders Ras(V12) cells capable for uncontrolled growth and metastatic behavior. Finally, chemical disruption of the lysosomal function by feeding animals with antimalarial drugs, chloroquine or monensin, leads to malignant tumor growth of the Ras(V12) cells. Taken together, our data provide evidence for a causative role of lysosome dysfunction in tumor growth and invasion and indicate that members of the Class C VPS complex behave as tumor suppressors.

Duke Scholars

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

July 9, 2010

Volume

285

Issue

28

Start / End Page

21817 / 21823

Location

United States

Related Subject Headings

  • ras Proteins
  • Vesicular Transport Proteins
  • RNA Interference
  • Neoplasms
  • Neoplasm Metastasis
  • Monensin
  • Models, Genetic
  • Lysosomes
  • Humans
  • Green Fluorescent Proteins
 

Citation

APA
Chicago
ICMJE
MLA
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Chi, C., Zhu, H., Han, M., Zhuang, Y., Wu, X., & Xu, T. (2010). Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. J Biol Chem, 285(28), 21817–21823. https://doi.org/10.1074/jbc.M110.131714
Chi, Congwu, Huanhu Zhu, Min Han, Yuan Zhuang, Xiaohui Wu, and Tian Xu. “Disruption of lysosome function promotes tumor growth and metastasis in Drosophila.J Biol Chem 285, no. 28 (July 9, 2010): 21817–23. https://doi.org/10.1074/jbc.M110.131714.
Chi C, Zhu H, Han M, Zhuang Y, Wu X, Xu T. Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. J Biol Chem. 2010 Jul 9;285(28):21817–23.
Chi, Congwu, et al. “Disruption of lysosome function promotes tumor growth and metastasis in Drosophila.J Biol Chem, vol. 285, no. 28, July 2010, pp. 21817–23. Pubmed, doi:10.1074/jbc.M110.131714.
Chi C, Zhu H, Han M, Zhuang Y, Wu X, Xu T. Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. J Biol Chem. 2010 Jul 9;285(28):21817–21823.

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

July 9, 2010

Volume

285

Issue

28

Start / End Page

21817 / 21823

Location

United States

Related Subject Headings

  • ras Proteins
  • Vesicular Transport Proteins
  • RNA Interference
  • Neoplasms
  • Neoplasm Metastasis
  • Monensin
  • Models, Genetic
  • Lysosomes
  • Humans
  • Green Fluorescent Proteins