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Slow histidine H/D exchange protocol for thermodynamic analysis of protein folding and stability using mass spectrometry.

Publication ,  Journal Article
Tran, DT; Banerjee, S; Alayash, AI; Crumbliss, AL; Fitzgerald, MC
Published in: Analytical chemistry
February 2012

Described here is a mass spectrometry-based protocol to study the thermodynamic stability of proteins and protein-ligand complexes using the chemical denaturant dependence of the slow H/D exchange reaction of the imidazole C(2) proton in histidine side chains. The protocol is developed using several model protein systems including: ribonuclease (Rnase) A, myoglobin, bovine carbonic anhydrase (BCA) II, hemoglobin (Hb), and the hemoglobin-haptoglobin (Hb-Hp) protein complex. Folding free energies consistent with those previously determined by other more conventional techniques were obtained for the two-state folding proteins, Rnase A and myoglobin. The protocol successfully detected a previously observed partially unfolded intermediate stabilized in the BCA II folding/unfolding reaction, and it could be used to generate a K(d) value of 0.24 nM for the Hb-Hp complex. The compatibility of the protocol with conventional mass spectrometry-based proteomic sample preparation and analysis methods was also demonstrated in an experiment in which the protocol was used to detect the binding of zinc to superoxide dismutase in the yeast cell lysate sample. The yeast cell sample analyses also helped define the scope of the technique, which requires the presence of globally protected histidine residues in a protein's three-dimensional structure for successful application.

Duke Scholars

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2012

Volume

84

Issue

3

Start / End Page

1653 / 1660

Related Subject Headings

  • Thermodynamics
  • Superoxide Dismutase
  • Ribonuclease, Pancreatic
  • Proteins
  • Protein Folding
  • Protein Binding
  • Myoglobin
  • Mass Spectrometry
  • Histidine
  • Deuterium Exchange Measurement
 

Citation

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Tran, D. T., Banerjee, S., Alayash, A. I., Crumbliss, A. L., & Fitzgerald, M. C. (2012). Slow histidine H/D exchange protocol for thermodynamic analysis of protein folding and stability using mass spectrometry. Analytical Chemistry, 84(3), 1653–1660. https://doi.org/10.1021/ac202927p
Tran, Duc T., Sambuddha Banerjee, Abdu I. Alayash, Alvin L. Crumbliss, and Michael C. Fitzgerald. “Slow histidine H/D exchange protocol for thermodynamic analysis of protein folding and stability using mass spectrometry.Analytical Chemistry 84, no. 3 (February 2012): 1653–60. https://doi.org/10.1021/ac202927p.
Tran DT, Banerjee S, Alayash AI, Crumbliss AL, Fitzgerald MC. Slow histidine H/D exchange protocol for thermodynamic analysis of protein folding and stability using mass spectrometry. Analytical chemistry. 2012 Feb;84(3):1653–60.
Tran, Duc T., et al. “Slow histidine H/D exchange protocol for thermodynamic analysis of protein folding and stability using mass spectrometry.Analytical Chemistry, vol. 84, no. 3, Feb. 2012, pp. 1653–60. Epmc, doi:10.1021/ac202927p.
Tran DT, Banerjee S, Alayash AI, Crumbliss AL, Fitzgerald MC. Slow histidine H/D exchange protocol for thermodynamic analysis of protein folding and stability using mass spectrometry. Analytical chemistry. 2012 Feb;84(3):1653–1660.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2012

Volume

84

Issue

3

Start / End Page

1653 / 1660

Related Subject Headings

  • Thermodynamics
  • Superoxide Dismutase
  • Ribonuclease, Pancreatic
  • Proteins
  • Protein Folding
  • Protein Binding
  • Myoglobin
  • Mass Spectrometry
  • Histidine
  • Deuterium Exchange Measurement